PMID: 6980967Jun 1, 1982Paper

Effect of cross-bridge kinetics on apparent Ca2+ sensitivity

The Journal of General Physiology
P W BrandtT Robinson

Abstract

Three different ways of shifting the pCa/tension curve on the pCa axis have been studied and related to changes in the rate constants of the cross-bridge cycle. The curve midpoint shifts to higher pCa's when the substrate (Mg-ATP) is reduced from 5 to 0.25 mM, when the phosphate concentration is reduced from 7.5 mM to 0, and when the ionic strength is reduced from 0.200 to 0.120. The Hill coefficients of the pCa/tension curve in our standard saline (5 mM substrate, 5 mM free ATP, 7.5 mM phosphate, ionic strength 0.200, 15 degree C) are between 5.1 and 5.6 and fall to 3.0 with the left shift of the curve brought about by reducing both substrate and phosphate. Left shifts of the curve produced by reduction in the ionic strength do not result ina lower Hill coefficient. Reducing eigher substrate or phosphate is associated with a reduction in the optimal frequency for oscillatory work, but reduction in ionic strength is not so associated. Maximum tension increases with the left shift of the curve brought about by reducing phosphate concentration or ionic strength, but tension decreases with the left shift of the curve accompanying substrate concentration reduction in phosphate-free saline. We argue that one mechanism for the observ...Continue Reading

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Citations

Aug 15, 1997·The Journal of Experimental Zoology·P Degn, H Gesser
Oct 1, 1992·Pflügers Archiv : European journal of physiology·L M GibsonD G Stephenson
Jun 1, 1983·Pflügers Archiv : European journal of physiology·G J StienenJ C Rüegg
Sep 1, 1987·Pflügers Archiv : European journal of physiology·P E HoarW G Kerrick
Feb 1, 1985·Biulleten' eksperimental'noĭ biologii i meditsiny·V I VekslerV I Kapel'ko
Jan 1, 2014·Archives of Biochemistry and Biophysics·Philip W Brandt, Corrado Poggesi
Feb 1, 1991·Quarterly Reviews of Biophysics·C C AshleyT J Lea
Mar 24, 2000·The Biochemical Journal·G A SmithJ I Vandenberg
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Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·J S Davis, M E Rodgers
Feb 1, 1986·The Journal of General Physiology·D T YueW G Wier
May 1, 1987·The Journal of General Physiology·R Ventura-ClapierG Vassort
Mar 1, 1988·The Journal of General Physiology·F V BrozovichA M Gordon
May 1, 1992·The Journal of General Physiology·D A Martyn, A M Gordon
Jul 23, 1998·Clinical and Experimental Pharmacology & Physiology·G H Rossmanith, O B Tjokorda
Apr 12, 2000·Circulation·R J HajjarJ K Gwathmey
Aug 31, 2004·Journal of Applied Physiology·Neeti PathareKrista Vandenborne

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