PMID: 8593941Mar 1, 1996Paper

Effect of cross-linking on matrix permeability. A model for AGE-modified basement membranes

Diabetes
J Boyd-White, J C Williams

Abstract

There is much evidence that basement membranes, such as in the renal glomerulus, act as macromolecular sieves, restricting the passage of proteins. Cross-linking of matrix proteins, as occurs because of advanced glycosylation end products (AGEs) in diabetes, may have an effect on the sieving properties of the basement membrane. To test this hypothesis, Matrigel, a basement membrane-like matrix, was cross-linked with glycolaldehyde and control and cross-linked matrices compared. Control matrices allowed less bovine serum albumin to pass through than did cross-linked matrices, with sieving coefficients (SCs) of 0.38 +/- 0.02 and 0.52 +/- 0.02, respectively (P < 0.0005). The control matrices also allowed less cross-linked albumin through than did the cross-linked matrices: 0.13 +/- 0.01 vs. 0.17 +/- 0.02 (P < 0.002). The SCs of a series of fluorescein isothiocyanate dextrans (four sizes, Mr 16,000- 168,000) were lower for the control matrix than for the cross-linked matrix (P < 0.03). In addition, the SC for glycated albumin (incubated with glucose-6-phosphate) was higher than that of normal albumin for both the control (P < 0.04) and cross-linked matrices (P < 0.001). These data indicate that cross-linking of the matrix increases...Continue Reading

Citations

Feb 12, 2010·Biomechanics and Modeling in Mechanobiology·Shih-Youeng ChuangThomas P Hedman
Oct 21, 1999·Microvascular Research·K KlaentschiJ A Brown
Sep 11, 2003·FEBS Letters·Anoop K PadivalRam H Nagaraj
Nov 29, 2005·Hearing Research·Susan T FrisinaRobert D Frisina
Mar 28, 2002·Biophysical Journal·Jeffrey A White, William M Deen
Mar 15, 2013·Clinical Science·Alan W StittTimothy M Curtis
May 23, 2012·Progress in Retinal and Eye Research·Jane Yellowlees DouglasMaria B Grant
Jul 20, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sharath SundararajThomas Hedman
May 12, 1998·The American Journal of Physiology·K KlaentschiJ E Tooke
Apr 9, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·B A WalshJ C Rutledge
Feb 29, 2000·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·D S RajM Levi

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