Effect of cross-linking on the physicochemical and in vitro properties of pullulan/dextran microbeads

Journal of Materials Science. Materials in Medicine
Soraya LanouarDidier Letourneur

Abstract

Hydrogels are very promising for tissue engineering as they provide scaffolds and a suitable microenvironment to control cell behavior and tissue regeneration. We used a patented method to obtain beads of pullulan/dextran cross-linked with sodium trimetaphosphate (STMP), that were already described for in vivo bone repair. The aim of this study was to provide a comparative analysis of microbeads made of polysaccharides prepared using three different STMP feeding ratio of 1.5, 2.25 or 3 % w/w. The morphology, swelling and biodegradability of these structures were assessed. Mesenchymal stem cells were also seeded to evaluate the cell organization onto the beads. We found that the amount of phosphorus resulting from the cross-linking was proportional to the introduced STMP concentration. An increase of cross-linking decreased the in vitro enzymatic degradability, and also decreased the swelling in PBS or water. The microstructures observed by SEM and confocal microscopy indicated that homogeneous spherical microbeads were obtained, except for the lower cross-linking ratio where the shapes were altered. Beads hydrated in PBS exhibited a mean diameter ranging from 400 to 550 µm with the decrease of STMP ratio. Cells adhered to the s...Continue Reading

References

Nov 1, 1976·Journal of Dental Research·R H Staat, C F Schachtele
Jan 21, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·I Gliko-KabirA Rubinstein
Jul 22, 2006·Biomaterials·Sylvain DevilleAntoni P Tomsia
Feb 27, 2007·Journal of Materials Science. Materials in Medicine·Noélie-Brunehilde ThébaudLaurence Bordenave
Jan 1, 2011·Annual Review of Chemical and Biomolecular Engineering·François BerthiaumeMartin L Yarmush
Apr 12, 2014·Theranostics·Amanda Karine Andriola SilvaCédric Chauvierre
Apr 11, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·J C FricainS Catros

❮ Previous
Next ❯

Citations

May 28, 2020·International Journal of Molecular Sciences·Marie-Noëlle LabourDidier Letourneur
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Teresa Simon-YarzaDidier Letourneur
Jun 4, 2019·Acta Biomaterialia·Jérôme GrenierDidier Letourneur

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.