Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period.

PloS One
Jorge BarriusoRafael P Mellado

Abstract

Bt-maize is a transgenic variety of maize expressing the Cry toxin from Bacillus turingiensis. The potential accumulation of the relative effect of the transgenic modification and the cry toxin on the rhizobacterial communities of Bt-maize has been monitored over a period of four years. The accumulative effects of the cultivation of this transgenic plant have been monitored by means of high throughput DNA pyrosequencing of the bacterial DNA coding for the 16S rRNA hypervariable V6 region from rhizobacterial communities. The obtained sequences were subjected to taxonomic, phylogenetic and taxonomic-independent diversity studies. The results obtained were consistent, indicating that variations detected in the rhizobacterial community structure were possibly due to climatic factors rather than to the presence of the Bt-gene. No variations were observed in the diversity estimates between non-Bt and Bt-maize. The cultivation of Bt-maize during the four-year period did not change the maize rhizobacterial communities when compared to those of the non-Bt maize. This is the first study to be conducted with Bt-maize during such a long cultivation period and the first evaluation of rhizobacterial communities to be performed in this transg...Continue Reading

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Citations

Mar 3, 2015·Research in Microbiology·Alessandra TurriniManuela Giovannetti
Apr 26, 2013·Molecular Ecology Resources·J Andrew DeWoodyJanna R Willoughby
Mar 19, 2013·PloS One·José R Valverde, Rafael P Mellado
Feb 10, 2016·Journal of the Science of Food and Agriculture·Amina YaqoobTayyab Husnain
Jul 11, 2018·World Journal of Microbiology & Biotechnology·Jingang LiangZhengguang Zhang

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Datasets Mentioned

BETA
SRA009281

Methods Mentioned

BETA
transgenic
electrophoresis
PCR

Software Mentioned

MEGAN
ESPRIT package
RDP
ESPRIT
BLASTN
Fast UniFrac
UniFrac
Infernal

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