Effect of cryopreservation techniques on aortic valve glycosaminoglycans

Artificial Organs
Luca DaineseGianluca Polvani

Abstract

This study was designed to evaluate the effect of cryopreservation on the glycosaminoglycan (GAG) content of the aortic allografts. Twenty-one porcine aortic valves were obtained. Five aortic roots were immediately analyzed without cryopreservation, eight were cryopreserved in closed leaflet position, and eight in open leaflet position. The groups were compared in terms of GAG concentration and subclass proportion in three different zones including the aortic root wall, the commissures, and the leaflets. GAG content at the commissures was significantly lower in the closed leaflet group than in the other groups (P = 0.001). The electrophoretic analysis did not show any significant difference in the zonal distribution of GAG classes between groups. Quantitative analysis in various aortic valve zones suggests that cryopreservation can alter the GAG content. Cryopreservation of the aortic valve in an open leaflet position can preserve the matrix more efficiently and might prolong the durability of the aortic allograft.

References

Jan 1, 1988·Cardiovascular Research·J D DeckS P Nolan
Aug 1, 1995·The Annals of Thoracic Surgery·R N MitchellF J Schoen
Apr 1, 1994·Cryobiology·Y H Shon, L Wolfinbarger
Nov 19, 1997·Journal of Korean Medical Science·Y H Shon
Feb 6, 1998·The Journal of Thoracic and Cardiovascular Surgery·R N MitchellF J Schoen
Jun 5, 2001·The Annals of Thoracic Surgery·E A Talman, D R Boughner
Sep 27, 2002·The Journal of Thoracic and Cardiovascular Surgery·David R KoolbergenJan A Bruijn
Nov 5, 2002·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Dave R KoolbergenRobert A E Dion
Apr 30, 2003·Transplantation·Kelvin G M Brockbank, Ying C Song
May 9, 2003·The Annals of Thoracic Surgery·Johanna J M TakkenbergAd J J C Bogers
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR
Mar 20, 2004·Expert Review of Cardiovascular Therapy·Helmut GulbinsB Reichart

❮ Previous
Next ❯

Citations

Mar 9, 2007·Artificial Organs·Paul S Malchesky
Dec 17, 2011·The Journal of Surgical Research·Padmini SarathchandraPatricia M Taylor
May 6, 2008·The Journal of Thoracic and Cardiovascular Surgery·Luca DainesePaolo Biglioli
Mar 31, 2012·Biochemistry Research International·Antonio CiglianoMarilena Formato
May 31, 2013·Journal of Biomechanical Engineering·Angela SeawrightBumsoo Han

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.