Effect of Cultural Conditions on Protease Production by a Thermophilic Geobacillus thermoglucosidasius SKF4 Isolated from Sungai Klah Hot Spring Park, Malaysia.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Allison D SuleimanNur Adeela Yasid

Abstract

Major progress in the fields of agriculture, industry, and biotechnology over the years has influenced the quest for a potent microorganism with favorable properties to be used in scientific research and industry. This study intended to isolate a new thermophilic-protease-producing bacterium and evaluate its growth and protease production under cultural conditions. Protease producing bacteria were successfully isolated from Sungai Klah Hot Spring Park in Perak, Malaysia, and coded as SKF4; they were promising protease producers. Based on microscopic, morphological, and 16S rRNA gene analysis, isolate SKF4 was identified as Geobacillus thermoglucosidasius SKF4. The process of isolating SKF4 to grow and produce proteases under different cultural conditions, including temperature, pH, NaCl concentration, carbon and nitrogen sources, and incubation time, was explored. The optimum cultural conditions observed for growth and protease production were at 60 to 65 °C of temperature, pH 7 to 8, and under 1% NaCl concentration. Further, the use of casein and yeast extract as the nitrogen sources, and sucrose and fructose as the carbon sources enhanced the growth and protease production of isolate SKF4. Meanwhile, isolate SKF4 reached maxi...Continue Reading

References

Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M B RaoV V Deshpande
Jun 19, 2001·FEMS Microbiology Letters·G C BakerA R Suharto
Jun 1, 2002·Current Opinion in Chemical Biology·Costanzo Bertoldo, Garabed Antranikian
Jun 19, 2002·Current Microbiology·Joseph F HawumbaVolker S Brözel
Nov 8, 2002·Systematic and Applied Microbiology·Teresa L MaugeriErko Stackebrandt
Jul 2, 2003·Current Opinion in Microbiology·Bertus van den Burg
Jan 1, 1965·Analytical Biochemistry·C E MCDONALD, L L CHEN
Jan 1, 1988·Biotechnology Advances·O P Ward, M Moo-Young
Mar 31, 2004·Canadian Journal of Microbiology·Marcus SchallmeyOwen P Ward
Aug 24, 2004·Extremophiles : Life Under Extreme Conditions·Xiao-Ge ChenStephen Tiong-Lee Tay
Nov 17, 2004·International Journal of Systematic and Evolutionary Microbiology·Tamara N NazinaGeorge A Osipov
Aug 19, 2005·The Journal of General and Applied Microbiology·Ida RomanoBarbara Nicolaus
Feb 4, 2006·Biodegradation·Christos MeintanisAmalia D Karagouni
Jan 20, 2009·Bioinformatics·Andrew M WaterhouseGeoffrey J Barton
Jun 1, 2017·Journal, Genetic Engineering & Biotechnology·Kanupriya Miglani SharmaAshwani Kumar
Mar 30, 2019·Food Technology and Biotechnology·Agnieszka KalwasińskaMaria Swiontek Brzezinska

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Citations

Oct 1, 2020·Journal of Food Biochemistry·Taiwo O AkanbiDominic Agyei
Jan 29, 2021·Applied Biochemistry and Biotechnology·Seden Güracar BaykaraGülşah Şanlı-Mohamed

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Datasets Mentioned

BETA
MN960021

Methods Mentioned

BETA
SMA
PCR

Software Mentioned

ClustalW
Basic Local Alignment Search Tools ( BLAST
Mega

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