Effect of culture conditions on ergosterol as an indicator of biomass in the aquatic hyphomycetes

Applied and Environmental Microbiology
J Y Charcosset, E Chauvet

Abstract

Ergosterol is a membrane component specific to fungi that can be used to estimate fungal biomass using appropriate factors of conversion. Our objectives were to determine the limits of use of ergosterol content as a measure of biomass for aquatic hyphomycetes, and to evaluate a previously established ergosterol-to-biomass conversion factor. We varied inoculum quality, growth medium, and degree of shaking of four aquatic hyphomycete species. In cultures inoculated with homogenized mycelium, we found a significant effect of shaking condition and culture age on ergosterol content. In liquid cultures with defined medium, ergosterol content reached 10 to 11 microg/mg of mycelium (dry mass) and varied by factors of 2.2 during exponential growth and 1.3 during stationary phase. The increase in ergosterol content during exponential phase could be attributed, at least in part, to rapid depletion of glucose. Oxygen availability to internal hyphae within the mycelial mass is also responsible for the differences found between culture conditions. Ergosterol concentration ranged from 0.8 to 1.6 microg/mg in static cultures inoculated with agar plugs. Ergosterol content varied by a factor of 4 in two media of different richnesses. For differe...Continue Reading

References

Feb 1, 1993·Applied and Environmental Microbiology·M O Gessner, E Chauvet
Oct 1, 1993·Applied and Environmental Microbiology·K SuberkroppE Chauvet

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Citations

Aug 23, 2007·Applied Microbiology and Biotechnology·Jian-Ping YuanXin Liu
Aug 4, 2009·Applied and Environmental Microbiology·Sofia DuarteJean-Yves Charcosset
Mar 4, 2000·Applied and Environmental Microbiology·K R Sridhar, F Bärlocher
Feb 8, 2013·Ecological Applications : a Publication of the Ecological Society of America·Thomas L BottJuliann M Battle
Sep 28, 2007·Journal of Applied Microbiology·X M FengD Rönnow
Mar 4, 2010·Proteomics·Thomas SchneiderKathrin Riedel
Sep 17, 2004·Journal of Microbiological Methods·Cecilia Mille-LindblomLars J Tranvik
Oct 9, 2004·International Journal of Hygiene and Environmental Health·Martin Hippelein, Melanie Rügamer
Jun 28, 2005·International Journal of Food Microbiology·Xin Mei FengJohan Schnürer
Apr 29, 2015·Phytotherapy Research : PTR·Zhi-Jian LiSilafu Aibai
Feb 1, 2011·FEMS Microbiology Reviews·Gerd-Joachim KraussFelix Bärlocher
May 18, 2016·The Science of the Total Environment·Diana BarrosFernanda Cássio
Sep 13, 2016·Frontiers in Microbiology·Gwenaëlle LashermesIsabelle Bertrand
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Oct 10, 2002·The Journal of Allergy and Clinical Immunology·Harriet A Burge

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