Effect of Culture in Simulated Microgravity on the Development of Mouse Embryonic Testes

Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University
Dorian NowackiMassimo De Felici

Abstract

All known organisms develop and evolve in the presence of gravitational force, and it is evident that gravity has a significant influence on organism physiology and development. Microgravity is known to affect gene expression, enzyme activity, cytoskeleton organization, mitotic proliferation and intracellular signaling. The aim of the present study was to study some aspects of the development in vitro of mouse embryonic testes in simulated microgravity. Testes from mouse embryos (12.5-16.5 days post coitum, d.p.c.) were cultured in simulated microgravity and standard static culture conditions. The microgravity condition was provided by a Rotary Cell Culture System (RWV) bioreactor, an apparatus designated for 3D tissue and small organ cultures. After 48 h of the culture in the RWV, testis morphology and size was evaluated. The first observation was that the culture in the RWV bioreactor had a beneficial effect on the testis growth and on the survival of germ cells in comparison to static 2D culture methods. Moreover, we found, that RWV culture caused disorganization the gonadal tissues, namely of the testis cords. The results suggest that the maintenance of testis cord could be sensitive to microgravity. We hypothesize that whi...Continue Reading

Citations

May 26, 2020·Reproduction, Fertility, and Development·Jie LiuBaohua Ma
Feb 25, 2020·The Journal of Veterinary Medical Science·Chinatsu HasegawaNobuhiko Hoshi
Jul 20, 2020·Journal of Assisted Reproduction and Genetics·M BoadaA Veiga

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Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.

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