Effect of culture temperature on a recombinant CHO cell line producing a C-terminal α-amidating enzyme.

Cytotechnology
K Furukawa, K Ohsuye

Abstract

In order to seek an efficient method for producing a recombinant protein by using animal cell culture, we investigated various effects of the culture temperature on a recombinant CHO cell line (3µ-1S), producing a C-terminal α-amidating enzyme (799BglIIα-AE) originating from Xenopus laevis. The results revealed that a low culture temperature (below 37 °C) led to the following phenomena: [1] inhibited cell growth, [2] enhanced cellular productivity of the recombinant protein, [3] maintained high cell viability, [4] suppressed medium consumption, and [5] suppressed release of impurities from the cells. These findings indicate that a quite simple method, the culture at low temperature, will contribute to the total improvement of the industrial process for the production of the recombinant protein, 799BglIIα-AE.

References

May 1, 1990·Bio/technology·C F Goochee, T Monica
Oct 15, 1990·Biochemical and Biophysical Research Communications·I KatoH Okamoto
Oct 29, 1987·Biochemical and Biophysical Research Communications·K MizunoH Matsuo
Jun 30, 1986·Biochemical and Biophysical Research Communications·K MizunoH Matsuo
Jan 22, 1986·Journal of Immunological Methods·S ReuvenyL Miller
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J Vilcek, E A Havell
Jul 1, 1980·Antimicrobial Agents and Chemotherapy·D J Giard, R J Fleischaker
Jun 1, 1982·Proceedings of the Society for Experimental Biology and Medicine·D J GiardM Fabricant
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B A EipperC C Glembotski
Aug 12, 1982·Nature·A F BradburyD G Smyth
Jan 1, 1994·Cytotechnology·R WeidemannG Kretzmer
Feb 5, 1991·Biotechnology and Bioengineering·G K Sureshkumar, R Mutharasan
Jul 1, 1992·Biotechnology and Bioengineering·J W BloemkolkT R Mosmann
Jul 1, 1993·Biotechnology and Bioengineering·A A LinW M Miller
Feb 1, 1988·Cytotechnology·O W Merten

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Citations

Feb 9, 2002·Biotechnology and Bioengineering·P DucommunI W Marison
Apr 6, 2004·Journal of Biotechnology·Mariela Bollati FogolínRicardo Kratje
Oct 24, 2003·Biotechnology and Bioengineering·Simone M SchatzFriedrich Scheiflinger
Feb 14, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Amin Ramezani, Abbas Ghaderi
Aug 7, 2008·Biotechnology and Bioengineering·Soo Hean Gary Khoo, Mohamed Al-Rubeai
Jun 11, 2019·Biotechnology and Bioengineering·Alina SchneiderOliver Popp
Jul 7, 2015·Biotechnology Progress·Jennifer PfizenmaierRalf Takors
Jul 9, 1999·Biotechnology and Bioengineering·H KaufmannJ E Bailey
Jun 24, 2006·The Biochemical Journal·Mohamed B Al-Fageeh, C Mark Smales
May 4, 2007·Trends in Biotechnology·Prateek Gupta, Kelvin H Lee

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