Effect of cycloheximide on palmitylation of PO protein of the peripheral nervous system myelin.

The Biochemical Journal
H C Agrawal, D Agrawal

Abstract

Incubation of rat sciatic nerve slices with Krebs-Ringer bicarbonate buffer containing [3H]palmitic acid resulted in the acylation of the PO glycoprotein and a 24 kDa protein of the peripheral nerve myelin. Radioactivity was removed from PO after treating PO with hydroxylamine (83%) and methanolic KOH (97%). These results provided evidence that the radioactivity incorporated into PO was not due to the metabolic conversion of [3H]palmitic acid into amino acids or sugars. PO was more heavily labelled in the homogenate than in the myelin membrane in 8-day-old rat nerve between 5 min and 2 h of incubation. These results suggested that PO may be primarily acylated in the cell body. Incubation of purified myelin with [1-14C]palmitoyl-CoA resulted in the non-enzymic acylation of PO. This provided evidence of the absence of fatty acyltransferase from the purified peripheral nerve myelin. Glycosylation of PO has been shown to occur in the Golgi complex, and monensin inhibited glycosylation of PO in the homogenate and myelin by 53 and 61% respectively. These results suggest that the processing of PO in the Golgi complex and the assembly of PO into myelin is impaired by monensin. However, fatty acylation of PO was unimpaired by monensin, ...Continue Reading

Citations

Jan 1, 1990·Annals of the New York Academy of Sciences·R M Gould
Jan 1, 1991·Acta neurochirurgica·C A PagniE Gaidolfi
Jul 4, 1995·International Journal of Cancer. Journal International Du Cancer·V A LiepkalnsC Icard-Liepkalns
Mar 1, 1994·Journal of Neurochemistry·O A BizzozeroA Pastuszyn
Dec 21, 2002·Pharmacology & Therapeutics·Riad Qanbar, Michel Bouvier
Jun 29, 1990·Biochemical and Biophysical Research Communications·H C AgrawalR H Quarles

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