Effect of cyclosporin A and ionophores on the intracellular pH of lymphocytes as measured by flow cytometry

Biochemical Medicine and Metabolic Biology
L TrónA Aszalos

Abstract

Because of the known importance of pHi changes on the biology of cells, we have investigated the effect of CsA, the clinically important immunomodulator, on the pHi of resting mouse spleen and human peripheral blood lymphocytes. We have found that pharmacological doses (0.8 microM) of CsA caused no change in the pHi in these cells. Similarly, the Ca2+ ionophore, A23187, up to 5 microns concentration, had no effect on the pHi of these cells, in contrast to previous reports. However, higher doses of CsA (2.4-8.0 microM) did change the pHi transiently or permanently. These results were obtained with flow cytometric measurements of fluorescence intensity of the pH-sensitive dye, BCECF.

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Citations

May 15, 1996·Journal of Biotechnology·P FranckP Nabet
Dec 1, 1992·Journal of Photochemistry and Photobiology. B, Biology·L BalkayL Trón
May 28, 1993·International Journal of Cancer. Journal International Du Cancer·J L WeaverA Aszalos

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