Effect of cysteamine on glutathione level and developmental capacity of bovine oocyte matured in vitro

Molecular Reproduction and Development
D de MatosH Baldassarre

Abstract

The present study was carried out to evaluate if the addition of cysteamine to the culture medium during in vitro maturation of bovine oocytes increased the glutathione (GSH) levels in the mature oocytes, and if these changes may promote an improvement on in vitro development to the blastocyst stage. Follicular oocytes from slaughterhouse ovaries were matured in TCM 199 supplemented with 10% (v/v) fetal calf serum, hormones, and O (control), 25, 50, or 100 muM of cysteamine for 24 hr. After in vitro maturation the oocytes were fertilized and cultured for 8 days. The percentage of embryos that developed to the blastocyst stage was significantly higher (P < 0.01) for oocytes matured in medium containing 100 muM of cysteamine than for those matured in control medium. Moreover, the intracellular GSH levels were increased (P < 0.05) in oocytes matured with 100 muM of cysteamine with respect to control. No differences were observed in maturation and cleavage rates, and in the mean cell numbers per blastocyst among treatments (P > 0.05). These results indicate that the addition of thiol compounds such as cysteamine to maturation medium increases the efficiency of in vitro blastocyst production from immature bovine oocytes. The higher ...Continue Reading

References

Jan 1, 1976·Annual Review of Biochemistry·A Meister, S S Tate
Jul 1, 1979·Physiological Reviews·B ChanceA Boveris
Jan 1, 1992·Molecular Reproduction and Development·M YoshidaV G Pursel
May 1, 1990·Molecular Reproduction and Development·N A TelfordG A Schultz
Sep 1, 1972·Journal of Reproduction and Fertility·H R TervitL E Rowson
Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Sep 3, 1984·Biochimica Et Biophysica Acta·S Bannai
Apr 29, 1983·Science·A Meister
Jul 1, 1994·Free Radical Research·M V LafleurJ Retèl
Jun 1, 1994·The International Journal of Biochemistry·A Del CorsoU Mura
May 1, 1993·Molecular Reproduction and Development·M Yoshida
Apr 1, 1986·Theriogenology·J J ParrishN L First

❮ Previous
Next ❯

Citations

Feb 1, 1997·Molecular Reproduction and Development·J L Edwards, P J Hansen
Feb 24, 2001·Theriogenology·B GasparriniL Zicarelli
Dec 25, 2002·Theriogenology·Y CogniéP Mermillod
Apr 17, 1999·European Journal of Obstetrics, Gynecology, and Reproductive Biology·M F KnapenE A Steegers
Sep 11, 2002·Animal Reproduction Science·A G MartínezG Brogliatti
May 7, 2010·Zygote : the Biology of Gametes and Early Embryos·Yoshikazu NagaoYoku Kato
Sep 14, 2001·Reproduction in Domestic Animals = Zuchthygiene·E GómezC Díez
Jan 22, 2004·Cloning and Stem Cells·B Oback, D N Wells
Mar 28, 2012·The Journal of Reproduction and Development·Masashi Takahashi
Sep 18, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Hua-Yu LianJing-He Tan
Feb 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Jason R HerrickRebecca L Krisher
Nov 26, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Jun-Zuo WangJing-He Tan
Dec 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Elena IbáñezDavid F Albertini
Apr 16, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Ping ZhouJing-He Tan
Aug 5, 2014·The Journal of Reproduction and Development·Robert E Click
Feb 5, 2008·Animal Reproduction Science·C C FurnusA L Errecalde

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.