Effect of cysteamine supplementation of in vitro matured bovine oocytes on chilling sensitivity and development of embryos

Animal Reproduction Science
S Balasubramanian, Gyu-Jin Rho

Abstract

In vitro techniques for production of bovine embryos including in vitro oocyte maturation (IVM), fertilization (IVF) and culture (IVC) are becoming increasingly employed for a variety of research purposes. However, decreased viability following cryopreservation by conventional methods has limited commercial applications of these technologies. A practical alternative to facilitate transport would be to arrest development by chilling without freezing. The present research was undertaken to evaluate chilling sensitivity of IVM-IVF embryos at different stages of development, and to determine possible beneficial effects of cysteamine treatment during IVM, previously shown to enhance embryo development in culture, on survival following chilling at different stages. Embryos produced by standard IVM-IVF-IVC methods were chilled to 0 degrees C for 30 min at 2-cell (30-34 h post-insemination, hpi), 8-cell (48-52 hpi) or blastocyst (166-170 hpi) stages. Viability after chilling was assessed by IVC with development to expanded blastocyst stage determined on days 7 and 8 post-insemination (pi) and hatching blastocyst stage determined on days 9 and 10 pi. Control embryos at the same stages were handled similarly, but without chilling, and de...Continue Reading

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Citations

Mar 10, 2010·Reproduction in Domestic Animals = Zuchthygiene·S Deleuze, G Goudet
May 3, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Teewara PhongnimitrRangsun Parnpai
May 1, 2015·Zygote : the Biology of Gametes and Early Embryos·Qing-Shan GaoXi-Jun Yin
Nov 7, 2016·Toxicology and Applied Pharmacology·Yandi WangLingjiang Min

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