Effect of D240G substitution in a novel ESBL CTX-M-27

The Journal of Antimicrobial Chemotherapy
R BonnetJ Sirot

Abstract

Escherichia coli clinical strain Gre-1 collected in 2000 from a French hospital harboured a novel CTX-M-encoding gene, designated blaCTX-M-27. CTX-M-27 differed from CTX-M-14 only by the substitution D240G and was the third CTX-M enzyme harbouring this mutation after CTX-M-15 and CTX-M-16. The Gly-240-harbouring enzyme CTX-M-27 conferred to E. coli higher MICs of ceftazidime (MIC, 8 versus 1 mg/L) than did the Asp-240-harbouring CTX-M-14 enzyme. Comparison of CTX-M-14 and CTX-M-27 showed that residue Gly-240 decreased Km for ceftazidime (205 versus 940 microM), but decreased hydrolytic activity against good substrates, such as cefotaxime (kcat, 113 versus 415 s-1), probably owing to the alteration of beta3 strand positioning during the catalytic process.

Citations

Apr 24, 2004·Canadian Journal of Microbiology·Jan Walther-Rasmussen, Niels Høiby
Jan 9, 2014·Annals of Clinical Microbiology and Antimicrobials·Mohamed H Al-AgamyHarish Khubnani
Sep 23, 2014·Médecine et maladies infectieuses·H LahlaouiM Ben Moussa
Oct 27, 2004·Antimicrobial Agents and Chemotherapy·Baha AbdalhamidNancy D Hanson
Nov 25, 2004·Antimicrobial Agents and Chemotherapy·Craig J MundayPeter M Hawkey
Jan 27, 2006·Antimicrobial Agents and Chemotherapy·J DelmasR Bonnet
Dec 3, 2008·Emerging Infectious Diseases·Maria SjölundJean M Whichard
May 1, 2009·Emerging Infectious Diseases·Etienne RuppéGuillaume Arlet
Jan 5, 2011·Emerging Infectious Diseases·Maria Sjölund-KarlssonJean M Whichard
Feb 24, 2011·Antimicrobial Agents and Chemotherapy·Laura DjamdjianPatrice Nordmann
Jul 7, 2011·Antimicrobial Agents and Chemotherapy·Francisco José Pérez-LlarenaGermán Bou
Aug 30, 2008·The Journal of Antimicrobial Chemotherapy·Giuseppe CelenzaMariagrazia Perilli
Mar 31, 2009·The Journal of Antimicrobial Chemotherapy·C ArpinUNKNOWN Scientific Committee of ONERBA
Jan 18, 2007·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Marisa CaccamoGianfranco Amicosante
Mar 8, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Wing Sze HoKwai Lin Thong
Oct 17, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Carlos RedondoGuillermina Alonso
Jan 8, 2013·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Maria Sjölund-KarlssonJean M Whichard
Aug 14, 2010·Foodborne Pathogens and Disease·Maria Sjölund-KarlssonJean M Whichard
Feb 19, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·A C CarvalhoA M F Cerqueira
Oct 5, 2007·Diagnostic Microbiology and Infectious Disease·Emilio David Valverde RomeroJuan Luis Muñoz Bellido
Jan 30, 2007·International Journal of Antimicrobial Agents·Soichiro KimuraKeizo Yamaguchi
Jan 25, 2005·International Journal of Antimicrobial Agents·Gioconda BriganteAntonio Toniolo
Nov 12, 2013·International Journal of Antimicrobial Agents·Yingfang WangQingtang Fan
Jun 16, 2012·Critical Reviews in Microbiology·Wei-Hua Zhao, Zhi-Qing Hu

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