Effect of Dandelion Extracts on the Proliferation of Ovarian Granulosa Cells and Expression of Hormone Receptors

Chinese Medical Journal
Tong WangYan-Min Ma

Abstract

In the current society, infertility related to age has become a social problem. The in vitro fertilization (IVF) success rate in women with poor ovarian response (POR) is very low. Dandelion extract T-1 (DE-T1) is an effective component of the extract from the leaves and stems of Taraxacum officinale, which is one of the medicines used in some patients with POR, but its molecular mechanism remains unclear. Following IVF, ovarian granulosa cells (GCs) of sixty patients were extracted and divided into normal ovarian response (NOR) and POR groups. GCs were cultured in a dose-dependent and time-dependent manner with DE-T1, proliferation of GCs was determined by Cell Counting Kit-8 assay, and mRNA levels of insulin-like growth factor 1 receptor (IGF-1R), luteotropic hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR), LHR, and CYP19A1 (aromatase) were determined by quantitative polymerase chain reaction. Progesterone and estradiol (E2) concentrations were determined by enzyme-linked immunosorbent assay. The cell viability gradually increased with the progressive increase in the DE-T1 concentration. Compared with the control group (without DE-T1), the mRNA expressions of FSHR, LHR, IGF-1R, and CYP19A1 were upregulate...Continue Reading

References

Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·A DierichP Sassone-Corsi
Jul 27, 2001·Molecular Human Reproduction·P ThiruppathiJ L Luborsky
Mar 13, 2002·The Journal of Clinical Endocrinology and Metabolism·Elaine DohertyKristiina Aittomäki
Dec 29, 2007·Animal Reproduction Science·O J GintherM A Beg
Apr 21, 2011·Human Reproduction·A P FerrarettiUNKNOWN ESHRE working group on Poor Ovarian Response Definition

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Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

BLAST
SPSS

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