Effect of deacetylation on the synergistic interaction of acetan with locust bean gum or konjac mannan

Carbohydrate Research
C OjinnakaV J Morris

Abstract

It has been discovered that deacetylation of the bacterial polysaccharide acetan promotes synergistic interactions with either locust bean gum (LBG) or konjac mannan (KM). Acetan is similar in structure to xanthan, and adopts a similar 5-fold conformation in the solid state. Like xanthan, it shows a thermally reversible order (helix)-disorder (coil) transition in solution. Both polymers have a cellulosic backbone with charged (anionic) sidechains attached at O-3 of alternate glucosyl residues, but the sidechains in acetan are longer (pentasaccharide rather than trisaccharide) and do not contain pyruvic substituents. Acetan has two sites of acetylation, one at O-6 of the inner mannosyl residue of the carbohydrate sidechains (as in xanthan) and the other on the polymer backbone (believed to be at O-6 of the branched glucosyl residues). Solutions of acetan or deacetylated acetan were equilibrated against 10 mM potassium chloride (to stabilise the ordered conformation) and were mixed (at 25 degrees C) with solutions of LBG or KM, also equilibrated against 10 mM potassium chloride. Unlike xanthan, native acetan showed no evidence of synergistic interaction with either LBG or KM. After deacetylation, however, large enhancements were ...Continue Reading

References

Feb 1, 1996·International Journal of Biological Macromolecules·M J RidoutV J Morris

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Citations

Dec 12, 2003·Carbohydrate Research·Rengaswami ChandrasekaranVictor J Morris
Mar 5, 2016·The Journal of Physical Chemistry. B·Makoto Takemasa, Katsuyoshi Nishinari
Apr 13, 2007·European Biophysics Journal : EBJ·C SandoloF Alhaique
Apr 19, 2007·Journal of Basic Microbiology·Anita Suresh KumarBhavanath Jha
May 20, 2000·Biotechnology & Genetic Engineering Reviews·I W Sutherland

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