Abstract
The sporulation of Bacillus subtilis can be induced in the presence of amino acids and glucose by partially depriving the cells of guanine nucleotides. This can be achieved, e.g., by the addition of decoyinine, a specific inhibitor of GMP synthetase. To determine the effect of this and other inhibitors on cell wall synthesis, we measured in their presence the incorporation of acetylglucosamine into acid-precipitable material. The rate of wall synthesis decreased by 50% within 5 min after decoyinine addition; this decrease was prevented by the presence of guanosine. A comparison with the effects of other inhibitors of cell wall synthesis indicated that decoyinine inhibited the final portion of the cell wall biosynthetic pathway, i.e., after the steps inhibited by bacitracin or vancomycin. Decoyinine addition also prevented cellular autolysis and cell wall turnover. It is not known whether these two effects of decoyinine on cell wall synthesis are causally related.
References
May 1, 1977·Journal of Bacteriology·L Glaser, B Lindsay
May 1, 1979·Journal of Bacteriology·D L ShunguG D Shockman
Oct 4, 1979·Biochimica Et Biophysica Acta·J M LopezE Freese
Nov 1, 1979·Journal of General Microbiology·E FreeseE M Galliers
Jul 1, 1978·Journal of Bacteriology·W D Ramey, E E Ishiguro
Jan 1, 1979·Advances in Microbial Physiology·H J Rogers
Sep 1, 1976·Journal of Bacteriology·E E Ishiguro, W D Ramey
Sep 1, 1976·Bacteriological Reviews·S Omura
Mar 1, 1976·Journal of Bacteriology·H M Pooley
May 1, 1973·Antimicrobial Agents and Chemotherapy·I GoldbergK Bloch
Mar 1, 1972·Journal of Bacteriology·D P FanW P Cunningham
Feb 1, 1973·Journal of Bacteriology·Y K OE Freese
Mar 1, 1970·Journal of Bacteriology·E B FreeseE Freese
Feb 20, 1970·Biochemical and Biophysical Research Communications·W C Brown, F E Young
Feb 1, 1971·Journal of Bacteriology·D P Fan, M M Beckman
Apr 1, 1981·Journal of Bacteriology·B Uratani-WongE Freese
Citations
Mar 1, 1990·Molecular Microbiology·B JorisJ M Ghuysen
Jan 1, 1987·Critical Reviews in Microbiology·R J Doyle, A L Koch
Aug 22, 2014·Microbiology·Mia RysselMogens Kilstrup
Apr 25, 2015·Cell Death and Differentiation·A Bianchi-SmiragliaM A Nikiforov
May 11, 2016·Journal of Bacteriology·Eric M KofoedMan-Wah Tan
Dec 1, 1988·Microbiological Reviews·R J DoyleV Vinter
Jun 1, 1985·Journal of Bacteriology·C E Buchanan
Dec 1, 1983·Journal of Bacteriology·H Y CheungE Freese
Aug 1, 1985·Journal of Bacteriology·Y Sadaie, T Kada