Effect of deletion of gra17 and gra23 genes on the growth, virulence, and immunogenicity of type II Toxoplasma gondii

Parasitology Research
Ting-Ting LiHany M Elsheikha

Abstract

The protozoan parasite Toxoplasma gondii secretes a number of dense granule proteins (GRAs) from the dense granule organelle to manipulate the host cell. Two of these effector proteins (GRA17 and GRA23) are involved in the trafficking of molecules between the parasitophorous vacuole (PV) and the host cell cytoplasm. However, their roles in establishing chronic infection remain obscured. In this study, CRISPR-Cas9 was used to delete gra17 or gra23 gene in T. gondii Pru strain (type II). The growth, the virulence, the ability to establish chronic infection, and the immunogenicity of the constructed mutant strains were investigated in Kunming mice. Pru:Δgra17 and Pru:Δgra23 mutants developed PVs with abnormal morphology and exhibited reduced growth rate, compared with the wild-type Pru strain. Deletion of gra17 abrogated acute infection and blocked cyst formation. Although the deletion of gra23 caused slight attenuation of the parasite virulence in mice, it caused a significant reduction in cyst formation. Immunization with Pru:Δgra17 induced high levels of IgG (IgG1 and IgG2a) antibodies and cytokines (interleukin-2 [IL-2], IL-10, IL-12, and interferon gamma [IFN-γ]), which conferred significant protection in mice challenged with...Continue Reading

References

Dec 1, 1995·The Journal of Infectious Diseases·D K Howe, L D Sibley
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Seon-Kyeong Kim, John C Boothroyd
Jan 5, 2011·The Journal of Experimental Medicine·Emily E RosowskiJeroen P J Saeij
Apr 12, 2012·Clinical Microbiology Reviews·Florence Robert-Gangneux, Marie-Laure Dardé
Apr 30, 2013·Molecular and Biochemical Parasitology·Tadashi OkadaMakoto Igarashi
Jan 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aditi AlagananL David Sibley
Jan 25, 2014·Nature Reviews. Immunology·Felix Yarovinsky
Apr 9, 2014·Infection and Immunity·Anjali J ShastriJohn C Boothroyd
Sep 17, 2014·The Journal of Experimental Medicine·Ji Su MaMasahiro Yamamoto
Jan 21, 2015·Trends in Parasitology·Corinne Mercier, Marie-France Cesbron-Delauw
Mar 24, 2017·Trends in Parasitology·Barbara Clough, Eva-Maria Frickel
Apr 25, 2017·Cellular Microbiology·Kaice A LaFaversGustavo Arrizabalaga
May 10, 2017·Trends in Parasitology·Elizabeth A WohlfertEmma H Wilson
Sep 14, 2018·Clinical Microbiology Reviews·Ildiko Rita DunayJose G Montoya
Sep 14, 2018·Frontiers in Cellular and Infection Microbiology·Meng-Jie BaiXing-Quan Zhu
Oct 2, 2019·Frontiers in Cellular and Infection Microbiology·Tatiana Paredes-SantosJeroen P Saeij
Jan 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jin-Lei WangXing-Quan Zhu
Jan 31, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin-Lei WangXing-Quan Zhu

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Citations

Nov 27, 2020·Clinical Microbiology Reviews·Hany M ElsheikhaXing-Quan Zhu
Dec 17, 2020·Frontiers in Cellular and Infection Microbiology·Marie-Noëlle MévélecIsabelle Dimier-Poisson
Apr 13, 2021·Frontiers in Cell and Developmental Biology·Natalie A CounihanTania F de Koning-Ward

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Methods Mentioned

BETA
GTPase
transfection
PCR
ELISA
enzyme-linked immunosorbent assay
ELISAs

Software Mentioned

SPSS18

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