PMID: 9635255Jun 23, 1998Paper

Effect of developmental age and hyperoxia exposure on kinase and phosphatase activities in newborn rat lungs

Experimental Lung Research
D M OlsonK M Eyster

Abstract

To better understand the biochemical events accompanying lung alveolarization and development, we studied the specific activity of the cAMP-dependent protein kinase (PKA) and the type 2A protein phosphastase (PP2A), and the activity and protein content of the calcium- and lipid-dependent protein kinase (PKC) in cytosolic preparations of lungs. Lungs were obtained from rat pups on day 2 of life and on days 7, 14, and 27 from pups exposed to hyperoxia (> 95% O2, days 4-14; 65% O2 days 15-27) or normoxia from day 4 onwards. There were no significant changes in PKA specific activity with developmental age or hyperoxic exposure. PKC specific activity increased significantly (P < .05) in normoxic animals from day 2 (64 +/- 13.5 pmol phosphate released/min/mg protein) to day 14 (105 +/- 9). The increase was sustained to day 27. There was no effect on PKC activity due to hyperoxia alone (ANOVA). This increase in PKC activity was accompanied by an increase in the mass of the delta, epsilon and zeta isoforms of PKC in normoxic pups. The gamma isoform of PKC was undetectable in all samples whereas the alpha and beta isoforms were detectable but showed no changes with developmental age. PP2A specific activity increased significantly (P < ....Continue Reading

References

Apr 30, 1990·Biochemical and Biophysical Research Communications·K M Eyster
Jul 1, 1988·Pediatric Research·D Warburton, P Cohen
Jan 1, 1985·Experimental Lung Research·J A WhitsettJ Lessard
Oct 1, 1985·The Journal of Clinical Investigation·D MassaroP Whitney
Dec 1, 1983·Pediatric Research·J A WhitsettC Darovec-Beckerman
Feb 1, 1994·Circulation Research·V O Rybin, S F Steinberg
Nov 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K M Eyster, D C McFarland
Feb 9, 1996·The Journal of Biological Chemistry·K AbeP C Maloney
Jan 1, 1996·Annual Review of Physiology·S R Hilfer
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·J S BurghardtD M Olson
Apr 1, 1997·Current Opinion in Cell Biology·A C Newton
Nov 14, 1997·Experimental Lung Research·M V ShethK M Eyster

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.