PMID: 9652398Jul 4, 1998Paper

Effect of dexamethasone on interleukin-1beta-(IL-1beta)-induced nuclear factor-kappaB (NF-kappaB) and kappaB-dependent transcription in epithelial cells

European Journal of Biochemistry
Robert NewtonP J Barnes

Abstract

The production of inflammatory mediators by epithelial cells in inflammatory lung diseases may represent an important target for the anti-inflammatory effects of glucocorticoids. Nuclear factor-kappaB (NF-kappaB) is a major activator of inflammatory genes and has been proposed as a target for inhibition by glucocorticoids. We have used human pulmonary type-II A549 and airway epithelial BEAS-2B cells to investigate the effect of glucocorticoids on NF-kappaB regulation and kappaB-dependent transcription. In A549 cells following interleukin-1beta (IL-1beta) treatment, there was no effect of dexamethasone on the disappearance of I kappaB alpha protein, its subsequent reappearance 90-min later or the rapid induction of I kappaB alpha mRNA and transcription rate. Expression of p65 and p50/p105 proteins were also unaffected by dexamethasone. In addition, the rapid IL-1beta-induction of NF-kappaB DNA binding and p65 nuclear localisation was unaffected by short (1-6 hours) dexamethasone pre-treatments. Similarly, BEAS-2B cells showed no effect of dexamethasone on IL-1beta-induced NF-kappaB (p50/p65). Stable transfection of a kappaB-dependent reporter in A549 cells resulted in an 8-9-fold activation by IL-1beta or phorbol ester, that was...Continue Reading

Citations

Mar 29, 2003·Current Allergy and Asthma Reports·Ian M Adcock
Jul 5, 2005·Biochemical Pharmacology·Anne-Sofie M JohanssonJan E W Palmblad
Oct 14, 2004·European Journal of Biochemistry·Joanna E ChiversRobert Newton
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Nov 8, 2008·American Journal of Respiratory Cell and Molecular Biology·Hisatoshi SugiuraMasakazu Ichinose
Jun 12, 2013·European Journal of Pharmacology·Robert Newton
Jul 29, 2009·Ophthalmic Surgery, Lasers & Imaging : the Official Journal of the International Society for Imaging in the Eye·E Jason Sabet-PeymanDiana V Do
Dec 13, 2007·The Journal of Allergy and Clinical Immunology·Cristiana Stellato
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Jan 8, 2015·Pharmacogenetics and Genomics·William J ChapinRong S Huang
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Apr 11, 2006·British Journal of Pharmacology·Peter J Barnes
Jan 31, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pankaj K BhavsarKian Fan Chung

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