Effect of diesel exhaust particles on mRNA expression of viral and bacterial receptors in rat lung epithelial L2 cells

Toxicology Letters
Tsuyoshi ItoKiyohisa Sekizawa

Abstract

Epidemiological studies have shown that particulate matter (PM) is associated with adverse respiratory health effects. Although infection in the respiratory organ is one of the most important health risks the association of infection with PM is not fully understood. As we had hypothesized that diesel exhaust particles (DEP), one of the major component of PM, may induce the expression of receptors for viruses and bacteria at invasion sites, we studied the effect of DEP on the mRNA expression of intercellular adhesion molecule-1 (ICAM-1), low-density lipoprotein (LDL) and platelet-activating factor (PAF) receptors, which are invasion sites of virus and bacteria, on rat lung epithelial cells. The real-time quantitative polymerase chain reaction (PCR) method was used for the evaluation. All of these mRNAs were up-regulated by 3, 10, and 30 microg/ml of DEP in a concentration-dependent manner. The up-regulation of each was associated with the mRNA expression of heme oxygenase-1 (HO-1), a marker of oxidative stress. Our present results show that DEP up-regulated the mRNA expression of viral and bacterial receptors. This up-regulation might be associated with DEP-induced oxidative stress. These results thus suggest that DEP may enhanc...Continue Reading

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Citations

Dec 12, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Hui-Juan CaoHiroshi Kurihara
Feb 14, 2008·The Journal of Infectious Diseases·Yi-Hsuan WuDaniel Tsun-Yee Chiu
May 6, 2015·International Journal of Molecular Medicine·Bo LiHan Jung Chae
Nov 11, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Naomi TsukueToru Nakajima
Jun 10, 2008·Toxicology Letters·Shannan RodriguezElizabeth Hong-Geller
Feb 5, 2008·Neuroscience Letters·Markus G KlassMarie Csete
Sep 4, 2007·Mutation Research·Pernille Høgh DanielsenPeter Møller
Sep 8, 2010·The Journal of Allergy and Clinical Immunology·Shuk-Mei Ho
Dec 21, 2006·Journal of Applied Toxicology : JAT·Ken-ichiro InoueToshikazu Yoshikawa
Oct 31, 2018·Respiratory Research·Dennis LoCynthia J Koziol-White
Nov 8, 2007·Inhalation Toxicology·Jonathan Ciencewicki, Ilona Jaspers
Oct 7, 2020·Annals of the New York Academy of Sciences·Brittany WoodbyGiuseppe Valacchi

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