Effect of diet quantity and urea supplementation on oocyte and embryo quality in sheep

Theriogenology
S PapadopoulosM P Bolan

Abstract

The objective was to investigate the effects of dietary energy and urea supplementation on oocyte and embryo quality in sheep using in vivo and in vitro experimental models. Sixty-three ewes were fed grass meal at 0.5 or 2.0 times maintenance energy requirements (MER). The diet was supplemented with feed grade urea (U) for half of the ewes on each energy treatment. Ewes were stimulated with 1000 IU eCG and either slaughtered on the day of pessary withdrawal, for in vitro embryo production, or mated and slaughtered on Day 5 for embryo recovery. Urea decreased cleavage rate (48.3 vs 39.7%) and consequently blastocyst rate (41.6 vs 36.8%) but the differences were not significant. Oocytes from animals on 2.0 MER had a lower cleavage rate (54.9 vs 36.0%) and blastocyst yield (49.3 vs 31.4%) than those on 0.5 MER. However, there was an interaction between urea and energy for cleavage (P = 0.04) and blastocyst yield (P = 0.03) indicating a variable response to urea in the presence of high energy. This was manifested by a decrease in cleavage rate in the presence of urea and high energy (22%, 8 of 36), and a reduction in blastocyst development (19%, 7 of 36). When blastocyst development rate was expressed as a proportion of cleaved ooc...Continue Reading

References

Feb 1, 1993·Biology of Reproduction·D K Gardner, M Lane
Aug 5, 2000·Animal Reproduction Science·G P ByrneM P Boland

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Citations

Aug 21, 2013·Animal Reproduction Science·M I VázquezJ A Abecia
Mar 8, 2012·Reproduction, Fertility, and Development·Tom P FlemingJudith J Eckert
May 23, 2014·Journal of Developmental Origins of Health and Disease·S Chadio, B Kotsampasi
Jan 1, 2013·Annual Review of Animal Biosciences·Rebecca L Krisher
Jan 20, 2005·Biology of the Cell·Keith E Latham
Mar 14, 2009·Reproduction in Domestic Animals = Zuchthygiene·M I VázquezI Palacín
May 14, 2016·Theriogenology·S BairagiL P Reynolds
Jun 17, 2011·Animal Reproduction Science·M A Velazquez
Jun 20, 2017·Reproduction in Domestic Animals = Zuchthygiene·C Naturil-AlfonsoF Marco-Jiménez
May 16, 2006·Journal of Animal Science·E BorowczykA T Grazul-Bilska
Mar 4, 2020·FASEB BioAdvances·Kouki ShiinaManabu Kawahara
Nov 10, 2020·Journal of Animal Science·Joel S CatonLawrence P Reynolds

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