Effect of dietary 7-ketocholesterol, pure, or oxidized cholesterol on hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity, energy balance, egg cholesterol concentration, and 14C-acetate incorporation into yolk lipids of laying hens

Poultry Science
R E VargasE C Naber

Abstract

Experiments were conducted to study the effect of 7-ketocholesterol (7-k) in the presence or absence of pure cholesterol (PCH) or oxidized cholesterol (OCH) in diets of laying hens on reproductive performance and several parameters of cholesterol metabolism. In the first experiment, cholesterol synthesis and transport was examined by the in ovo incorporation of 14C-acetate into yolk triglycerides and cholesterol. Energy balances were also conducted. In the second experiment, hepatic 3 hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity was measured in vitro to evaluate potential cholesterol synthesis. In both experiments, reproductive performance and egg yolk cholesterol concentration were measured. Dietary PCH or OCH (.5%) significantly reduced relative acetate incorporation into yolk cholesterol, while 7-k (.025%) had no effect on carbon flow from acetate into egg cholesterol. While 7-k alone did not alter total yolk cholesterol concentration, it moderated the effect of PCH or OCH on increasing yolk cholesterol concentration. No consistent effects of dietary sterols on reproductive performance or energy balance were observed. Hepatic HMG CoA reductase activity was dramatically suppressed by feeding PCH or OCH and...Continue Reading

Citations

Mar 25, 1992·Biochimica Et Biophysica Acta·A Kuksis
Jan 1, 1989·Free Radical Biology & Medicine·L L Smith, B H Johnson
Nov 1, 1990·Clinical Physics and Physiological Measurement : an Official Journal of the Hospital Physicists' Association, Deutsche Gesellschaft Für Medizinische Physik and the European Federation of Organisations for Medical Physics·J A Bushman
Jan 5, 2000·Physiological Reviews·G J Schroepfer

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