Effect of dietary calcium and cholic acid on the proliferative indices of murine colonic epithelium

Carcinogenesis
R P BirdW R Bruce

Abstract

The effect of dietary calcium on the cholic acid-induced colon cell proliferative response was investigated with C57BL/6J mice. The control diets were formulated on the basis of the AIN-76 diet with the levels of calcium set at 0.1, 0.5 or 1.0% by weight and the ratio of Ca:P at approximately 1:1. Cholic acid was added at 0.1% and 0.25% by weight. The cytokinetic parameters of the colons were evaluated after 2 weeks of feeding by the metaphase arrest technique and by autoradiography. Mitotic activity was found to monotonically decrease with increasing calcium level from 0.1 to 1.0% for both the cholic acid and control groups, cholic acid-fed groups exhibiting significantly higher mitotic activity than the control groups. The effect of calcium (0.1 versus 1.0%) was evident throughout the 24-h period with both the control and cholic acid-supplemented diets. Autoradiographic analyses of the colons yielded similar results. Among the controls, the 0.1% Ca group exhibited significantly higher (P less than or equal to 0.05) crypt height, higher whole crypt labelling index and higher labelling indices at various positions along the crypt height than the 1.0% Ca group. Cholic acid-fed groups had higher values for crypt height, whole cry...Continue Reading

Citations

Jan 1, 1994·Journal of Cellular Biochemistry. Supplement·G J KelloffG G Knapp
Nov 22, 1997·Diseases of the Colon and Rectum·R Adell-CarcellerJ Ruiz-Castillo
Dec 1, 1988·Mutation Research·H HayatsuT Negishi
Apr 1, 1990·Medical Hypotheses·D A McCarronR P Heaney
Jun 6, 2000·Mutation Research·T M de Kok, J M van Maanen
Jan 1, 1988·Nutrition and Cancer·V E Archer
Jan 1, 1991·Nutrition and Cancer·G CaderniD Kriebel
Apr 2, 1997·Journal of the National Cancer Institute·B S ReddyC V Rao
Jan 18, 2007·Journal of the National Cancer Institute·Maria V GrauJack S Mandel
Jan 1, 1990·Journal of Gastroenterology and Hepatology·C B Williams, L Bedenne
Oct 29, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Veronika FedirkoJill Joelle Woodard
Aug 26, 2009·Nutrition Reviews·Jennifer R PearsonIan R Rowland
May 1, 1990·Mutation Research·M J Hill
Nov 9, 2006·Molecular Carcinogenesis·Christopher FlynnDaniel W Rosenberg
Aug 1, 1987·Journal of Cellular Physiology·P M EcklR L Jirtle
Jan 1, 1996·Toxicologic Pathology·M B Thompson
Sep 15, 1992·Cancer·B Levin
Jan 1, 1991·Scandinavian Journal of Gastroenterology. Supplement·J W WelbergE G De Vries
Apr 17, 1987·Science·B N AmesL S Gold

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