Effect of dietary gluten on dendritic cells and innate immune subsets in BALB/c and NOD mice

PloS One
Jesper LarsenKarsten Buschard

Abstract

The innate immune system is known to play an important role in oral tolerance to dietary antigens. This is important in development of celiac disease (CD) but may also be important in type 1 diabetes (T1D), and could potentially explain the reduced incidence of T1D in mice receiving a gluten-free (GF) diet. The direct in vivo effect of gluten on innate cells, and particularly dendritic cells (DC) is not sufficiently clarified. Therefore, we wished to investigate the innate cell populations of spontaneous diabetic NOD mice and healthy BALB/c mice kept on a GF or a standard (STD) gluten containing diet. We studied, by flow cytometry and reverse transcription-quantitative polymerase chain reaction (qRT-PCR), if dietary gluten induces changes in the activation of DCs and distribution of selected innate cells in lymphoid, pancreatic and intestinal tissues in BALB/c and NOD mice. We found that a GF diet increased the percentage of macrophages in BALB/c spleen and of CD11c+ DCs in BALB/c and NOD spleen. Strictly gluten-free (SGF) diet increased the percentage of CD103+ DCs in BALB/c mice and decreased percentages of CD11b+ DCs in mesenteric and pancreatic lymph nodes in BALB/c mice. SGF diet in BALB/c mice also decreased DC expression...Continue Reading

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Citations

Jul 21, 2015·Autoimmunity Reviews·Aaron Lerner, Torsten Matthias
May 12, 2016·Current Diabetes Reports·Rene J McLaughlinBart O Roep
Nov 1, 2016·Journal of Diabetes Research·Susanne W BruunKarsten Buschard
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Nov 13, 2015·Nutrients·María E Mejía-León, Ana M Calderón de la Barca
Aug 23, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Justine M Abais-Battad, David L Mattson
Dec 1, 2018·The EMBO Journal·Valeria R VillellaLuigi Maiuri
Nov 16, 2018·Nutrients·Martin Haupt-JorgensenKarsten Buschard
Mar 14, 2021·Cell Death Discovery·Mara GagliardiMarco Corazzari
Dec 10, 2019·Journal of Agricultural and Food Chemistry·Clélia VilleminGrégory Bouchaud
Oct 12, 2017·Pediatrics·Christine T Ferrara, Stephen E Gitelman

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Methods Mentioned

BETA
flow cytometry
FACS

Software Mentioned

Primer3

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