Effect of different antibiotics on biofilm produced by uropathogenic Escherichia coli isolated from children with urinary tract infection

Pathogens and Disease
María José GonzálezPaola Scavone

Abstract

Recurrent urinary tract infections (UTIs) occur frequently in children and women. Intracellular bacterial communities (IBCs) and biofilm formation by Escherichia coli are risk factors for recurrence. The aim of this study was to evaluate the effect of different antibiotics on biofilms by E. coli strains isolated from children with UTI and to correlate virulence factors and IBCs with biofilm formation. A total of 116 E. coli strains were tested for biofilm formation using the crystal violet microplate technique. 58.6% of the strains did not produce biofilm, while 16.4%, 18.1% and 6.8% formed weak, moderate and strong biofilms, respectively. No correlation was found between the ability to form biofilms and the presence of IBCs. Biofilm formation was significantly associated with pili P codifying genes, whereas other virulence factors were not statistically associated. Antibiotics, including ampicillin, cephalothin, ceftriaxone, ceftazidime, amikacin and ciprofloxacin, were evaluated at different concentrations after 48 h of biofilm formation. Except ampicillin, the other antibiotics tested induced a significant reduction of biofilm biomass. In the case of recurrent UTIs potentially associated with the presence of biofilm, the use...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 5, 2002·Clinical Microbiology Reviews·Rodney M Donlan, J William Costerton
Aug 27, 2002·Emerging Infectious Diseases·Rodney M Donlan
Jun 7, 2003·Molecular Microbiology·Mikkel KlausenTim Tolker-Nielsen
Jul 5, 2003·Science·Gregory G AndersonScott J Hultgren
Jan 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sheryl S JusticeScott J Hultgren
Sep 1, 2004·Trends in Microbiology·Gregory G AndersonScott J Hultgren
Oct 15, 2005·Clinical Microbiology Reviews·David L Paterson, Robert A Bonomo
Sep 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Indira U Mysorekar, Scott J Hultgren
Apr 10, 2007·Journal of Bacteriology·Katherine P LemonRoberto Kolter
May 30, 2007·Research in Microbiology·Christine M ToutainGeorge A O'Toole
Dec 21, 2007·PLoS Medicine·David A RosenScott J Hultgren
Mar 28, 2008·Journal of Applied Microbiology·P NavesF Soriano
Jun 2, 2009·Current Microbiology·Iram LiaqatAnjum Nasim Sabri
Nov 18, 2009·Infection and Immunity·Kelsey E Sivick, Harry L T Mobley
Mar 17, 2010·Antimicrobial Agents and Chemotherapy·Matthew G Blango, Matthew A Mulvey
Aug 3, 2010·Nature Reviews. Microbiology·Hans-Curt Flemming, Jost Wingender
Nov 18, 2011·Science Translational Medicine·Corinne K CusumanoScott J Hultgren
Mar 16, 2012·The New England Journal of Medicine·Thomas M Hooton
Aug 14, 2012·International Journal of Pediatrics·Sabeen Habib
Nov 20, 2012·Frontiers in Cellular and Infection Microbiology·Susan R SteyertDavid A Rasko
Nov 22, 2012·The Journal of Antibiotics·Saloni SinglaSanjay Chhibber
Jan 30, 2013·BMC Infectious Diseases·Carlos J SanchezClinton K Murray
Dec 11, 2013·TheScientificWorldJournal·Natalia Angel VillegasMaria Paraje
Aug 6, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Luciana RobinoRafael Vignoli
Jun 10, 2015·Pediatrics·Ron KerenAlejandro Hoberman
Nov 7, 2015·Microbiology Spectrum·Peter Chahales, David G Thanassi

❮ Previous
Next ❯

Citations

Jan 3, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Paula Da CundaPaola Scavone
Oct 18, 2019·Antibiotics·Karen Arely Zapién-ChavarríaGuadalupe Virginia Nevárez-Moorillón
Jan 31, 2020·Frontiers in Microbiology·Elodie OlivaresFrançois Jehl
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·María José GonzálezLuciana Robino

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.