Effect of different cellulase dosages on cell viability and ethanol production by Kluyveromyces marxianus in SSF processes

Bioresource Technology
E Tomás-PejóMercedes Ballesteros

Abstract

This study was aimed to study the effect of commercial cellulases (Celluclast 1.5 LFG) on Kluyveromyces marxianus CECT 10875 growth and ethanol production in SSF processes. Preliminary tests carried out in glucose (50 g/L) fermentation medium showed that high enzyme amounts (2.5-3.5 FPU/mL) could cause a negative effect on K. marxianus growth rate and viable cells number. However, the maximum ethanol production was not affected and about 86% of the theoretical (22 g/L) was reached in all cases independently of the enzyme dosage. In SSF experiments, cell viability was always affected by enzyme loading. Nevertheless, slight differences observed on cell viability during glucose fermentation processes with the detected concentrations of the additives did not justify the negative effect observed in SSF experiments.

References

Feb 1, 2000·Applied Biochemistry and Biotechnology·C M TakahashiF Alterthum
Oct 8, 2004·Biotechnology and Bioengineering·Enikõ VargaAnne Belinda Thomsen
Dec 14, 2004·Bioresource Technology·Nathan MosierMichael Ladisch

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Citations

May 15, 2010·Applied Microbiology and Biotechnology·Takao KitagawaTakao Imaeda
Feb 19, 2014·Applied Microbiology and Biotechnology·Daniela A CostaLuciano G Fietto
Dec 3, 2014·Bioresource Technology·Alfredo Oliva-TaravillaMercedes Ballesteros
Nov 20, 2013·Applied Microbiology and Biotechnology·Sanjay KumarAnil Kumar Puniya
Mar 8, 2017·Biotechnology for Biofuels·Constantinos KatsimpourasEvangelos Topakas

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