Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen

Animal Reproduction Science
A A TarigM Ebrahimi

Abstract

The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) combined with different percentages of egg yolk in Tris extender on the quality of chilled and frozen-thawed bull semen. A total of 24 ejaculates from four bulls were collected using an electroejaculator. Semen samples were diluted with 8% VCO in Tris extender which contained different concentrations 0% (control), 4%, 8%, 12%, 16% and 20% egg yolk. The diluted semen samples were divided into two fractions: one was chilled and stored at 4°C until evaluation after 24, 72, and 144h; the second fraction was processed by chilling for 3h at 4°C to equilibrate, then packaged in 0.25ml straws and frozen and stored in liquid nitrogen at -196°C until evaluation after 7 and 14 days. Both chilled and frozen semen samples were then thawed at 37°C and assessed for general motility using computer-assisted semen analysis (CASA), viability, acrosome integrity, and morphology (eosin-nigrosin), membrane integrity (hypo-osmotic swelling test) and lipid peroxidation (thiobarbituric acid-reactive substances (TBARS)). The results indicate treatments with 8%, 12%, 16% and 20% egg yolk with 8% VCO had greater sperm quality (P<0.05) as compared with the control. The treatme...Continue Reading

Citations

Aug 25, 2017·Zygote : the Biology of Gametes and Early Embryos·J M Morrell, I Mayer
Sep 26, 2019·Frontiers in Veterinary Science·Muhammet Rasit UgurErdogan Memili
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Hongtao WangJun Wang
Jul 6, 2021·Veterinary World·Ghadeer Sabah Bustani, Falah Hasan Baiee
Aug 7, 2021·Biology·Aitor Fernandez-NovoSonia Salome Perez-Garnelo

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