Effect of different glycerol levels on the release of glutamic oxaloacetic transaminase (GOT) from deep frozen ram spermatozoa.

Andrologia
P N RoychoudhuryP K Pareek

Abstract

Release of GOT was determined during different treatments in ram semen extended (1:10) with lactose-egg yolk diluter containing 3.5% and 5% glycerol (v/v). Semen samples were collected by the electro-ejaculation technique and GOT was analysed colorimetrically. Highly significant difference (p less than 0.01) was found in the post-thaw percent motile spermatozoa and GOT release (IU/10(9) spermatozoa) between 3.5% and 5% glycerol containing equilibrated samples. The difference in percent motile spermatozoa was not significant between 3.5% and 5% glycerol levels in control, post-thaw unequilibrated and prefreeze equilibrated samples. Whereas the difference in GOT release between 3.5% and 5% glycerol concentrations was not significant in control and post-thaw unequilibrated samples and highly significant (p less than 0.01) in prefreeze equilibrated samples.

References

Apr 1, 1974·Journal of Reproduction and Fertility·S Salamon, D Visser
Sep 1, 1967·Cryobiology·E F Graham, M M Pace
Mar 1, 1969·Journal of Reproduction and Fertility·P J QuinnK W Cleland
Feb 1, 1969·Journal of Reproduction and Fertility·P Healey
Jul 1, 1957·American Journal of Clinical Pathology·S REITMAN, S FRANKEL

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Citations

Dec 8, 2015·Journal of Animal Science·B WestfalewiczA Ciereszko
Jun 1, 1979·Zentralblatt für Veterinärmedizin. Reihe A·G P ChinnaiyaN C Ganguli
Jun 25, 2020·Animals : an Open Access Journal From MDPI·Valentina LongobardiBianca Gasparrini

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