Effect of different methods of cryopreservation on the cytoskeletal integrity of dromedary camel (Camelus dromedarius) embryos

Animal Reproduction Science
J A SkidmoreT A E Stout

Abstract

This study examined the effect of different methods of cryopreservation on the cytoskeletal integrity of camel embryos. A total of 32 embryos were recovered on Days 6 and 7 after ovulation and measured before being frozen using either a conventional slow-cooling technique (n=12: six Day 6 and six Day 7 embryos) or vitrification (n=12: four Day 6 and eight Day 7). The remaining 8 'control' embryos (four Day 6 and four Day 7) were not cryopreserved but instead incubated in holding medium for 30 min. After thawing, warming or incubation, the embryos were stained with 4,6-diamino-2-phenylindole dihydrochloride (DAPI) to identify dead cells. Subsequently, the embryos were fixed in 4% paraformaldehyde, permeabilized and labelled with Alexa Fluor 488-Phalloidin to enable assessment of cytoskeleton integrity. Vitrified-warmed embryos contained a significantly higher percentage of dead cells than either conventionally frozen embryos or controls (P<0.05). Although the proportion of dead cells in conventionally frozen embryos tended to be higher than in controls, the difference was not significant (P> or =0.07). Whereas embryo size did not affect the number of dead cells in conventionally frozen embryos, vitrified-warmed embryos >300 micr...Continue Reading

References

Aug 26, 1998·Molecular Reproduction and Development·G VajtaH Callesen
Dec 7, 2000·Zygote : the Biology of Gametes and Early Embryos·W H WangB N Day
Jun 8, 2002·Reproduction, Fertility, and Development·Julian A SkidmoreW R Allen
Sep 11, 2002·Animal Reproduction Science·J F AllerR H Alberio
Jun 1, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·T TharasanitT A E Stout

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Citations

Oct 5, 2013·Medical Science Monitor Basic Research·Razif DasimanNor-Ashikin Mohamed Noor Khan
Nov 17, 2012·Animal Reproduction Science·Abdelhaq Anouassi, Ahmed Tibary
Jul 17, 2018·Reproduction in Domestic Animals = Zuchthygiene·Raphael da Silva CostaAlexandre Ninhaus-Silveira

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