PMID: 3768169Aug 1, 1986Paper

Effect of different neutral phospholipids on apolipoprotein binding by artificial lipid particles in vivo

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
M F Tong, A Kuksis

Abstract

Soybean triacylglycerol particles, stabilized with egg yolk sphingomyelin (SPH), phosphatidylcholine (PC), phosphatidylethanolamine (PE), or PC-PE mixtures, with diameters ranging from 170 to 550 nm were prepared by sonication and isolated by ultracentrifugation. Binding of apoproteins to the lipid particles was studied in vivo using the strategy of Connelly and Kuksis. The recoveries of the injected particles, which had decreased in size and undergone minimal changes in lipid composition, ranged from 70% and 57% for SPH- and PC-stabilized particles to 14% for particles stabilized with egg yolk PC-PE mixtures. The apoprotein (apo) composition of the recovered particles showed qualitative and quantitative differences, which were affected by the number of washes during isolation. After four washes, the SPH and PC particles contained apoE, apoC-II, and apoC-III as major components and apoA-IV as minor components. In addition, all particles, except those stabilized with egg yolk PC, contained large amounts of albumin. In contrast to egg yolk PC, the dipalmitoyl PC particles bound albumin as a major component. The recovered PC-PE and PE particles were characterized by a relative decrease of apoC and greatly increased binding of albu...Continue Reading

Citations

Sep 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Maxwell A RubyRonald M Krauss
Jul 21, 2015·Biochimica Et Biophysica Acta·Peng Yang, Papasani V Subbaiah

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