Effect of different thawing rates on post-thaw sperm viability, kinematic parameters and motile sperm subpopulations structure of bull semen

Animal Reproduction Science
R MuiñoA Peña

Abstract

The aim of the present study was to evaluate three thawing rates for bull semen frozen in 0.25-ml straws: placing the straws in a water bath at 37 degrees C for 40s, at 50 degrees C for 15s or at 70 degrees C for 5s. In a first experiment, the three thawing rates were compared in relation to post-thaw sperm motility, determined subjectively, and sperm plasma and acrosomal membrane integrity, examined by flow cytometry, after 0 and 5h of incubation at 37 degrees C. In a second experiment, the three thawing rates were evaluated based on post-thaw sperm motility, determined using a CASA system, after 0 and 2h of incubation at 37 degrees C. In addition, for the motile spermatozoa, the individual motility descriptors were analysed using a multivariate clustering procedure to test the presence of separate sperm subpopulations with specific motility characteristics in the thawed bull semen samples. Finally, it was investigated if the thawing rate had any influence on the relative frequency distribution of spermatozoa within the different subpopulations. In terms of overall post-thaw motility or plasma and acrosomal sperm membrane integrity there were no significant differences between the three thawing methods evaluated. The statistic...Continue Reading

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Citations

Jul 4, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Alberto ContriAugusto Carluccio
Nov 26, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Wei SiJohn K Critser
Dec 15, 2012·Zygote : the Biology of Gametes and Early Embryos·S S M FaezahS I Fatimah
Mar 1, 2011·Animal Reproduction Science·Diego Gouveia SouzaTereza C Cardoso
May 11, 2010·Theriogenology·Alberto ContriAugusto Carluccio
Mar 7, 2014·Reproduction in Domestic Animals = Zuchthygiene·C TomásE de Mercado
Jan 12, 2011·Theriogenology·Felipe Martínez-PastorPaulino de Paz
May 10, 2016·Journal of Animal Science and Biotechnology·Alessia GloriaAlberto Contri
Jul 25, 2013·Reproduction in Domestic Animals = Zuchthygiene·I Rodríguez-LozanoA M Rosales-Torres
Sep 26, 2020·Scientific Reports·Nicolás ChiaranteSilvina Perez-Martinez
Oct 31, 2017·The Journal of Reproduction and Development·Iulian IbănescuHeinrich Bollwein
Aug 2, 2018·Journal of Plant Research·Taisuke TogawaKatsuyuki T Yamato
Dec 11, 2020·Theriogenology·Suzane Peres CampanholiLindsay Unno Gimenes
May 21, 2021·Reproduction in Domestic Animals = Zuchthygiene·Myrian Megumy Tsunokawa HidalgoMaria Isabel Mello Martins

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