Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress.

Journal of Veterinary Science
Mi Na LeeHo Jae Han

Abstract

Oxidative stresses induced by reactive oxygen species (ROS) have been shown to be involved in several physiological and pathophysiological processes, such as cell proliferation and differentiation. Steroid hormones can protect cells against apoptosis or induce cell proliferation by several mechanisms. Among androgenic hormones, dihydrotestosterone (DHT) is generated by a 5alpha- reduction of testosterone. Unlike testosterone, DHT cannot be aromatized to estradiol, therefore DHT is considered a pure androgenic steroid. This study was conducted to examine the effect of DHT (10(-7) M) on H2O2 (10(-3) M) -induced injuries in mouse embryonic stem (ES) cells. H2O2 induced ROS generation and increased lipid peroxide formation and DNA fragmentation. These effects of H2O2 were inhibited by pretreatment with DHT. H2O2 also increased the phosphorylation of p38 MAPK, SAPK/JNK and nuclear factor kappa B (NF-kappaB), but DHT blocked these effects. Moreover, H2O2 decreased DNA synthesis and the levels of cell cycle regulatory proteins [cyclin D1, cyclin E, cyclin-dependent kinase (CDK) 2, and CDK 4]. These effects of H2O2 were inhibited by pretreatment with DHT. In conclusion, DHT may partially prevent H2O2-induced cell injury through inhibit...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Sep 30, 1991·The American Journal of Medicine·C G Cochrane
Apr 1, 1990·Photochemistry and Photobiology·A W Girotti
Jan 1, 1990·Free Radical Biology & Medicine·Y Sun
Jan 1, 1988·Free Radical Biology & Medicine·P A WardK J Johnson
Jul 14, 1995·The Journal of Biological Chemistry·M Karin
May 15, 1995·Genes & Development·C J Sherr, J M Roberts
Jun 23, 1995·The Journal of Biological Chemistry·M H Cobb, E J Goldsmith
Feb 24, 1995·Cell·D Thanos, T Maniatis
Jun 21, 1996·The Journal of Biological Chemistry·J A KazzazS Horowitz
Oct 4, 1996·Cell·P A Baeuerle, D Baltimore
Jan 1, 1997·Journal of the National Cancer Institute·M O RippleG Wilding
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·F A Brook, R L Gardner
Sep 1, 1997·The Prostate·A M BakerM B Cohen
Apr 30, 1998·Current Opinion in Cell Biology·Y T Ip, R J Davis
May 20, 1999·The Anatomical Record·K S O'Shea
Sep 22, 2001·Cell Structure and Function·H Niwa
Dec 14, 2002·Anatomia, histologia, embryologia·Katja PrelleEckhard Wolf
Feb 7, 2003·Mini Reviews in Medicinal Chemistry·Eugenio FloresIvonne Heuze
Feb 26, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ronald WolfUlrike Blume-Peytavi
Mar 27, 2003·Urology·Culley Carson, Roger Rittmaster
May 12, 2004·Current Medicinal Chemistry·G PoliE Chiarpotto
Jul 6, 2004·Molecular Reproduction and Development·A Gutiérrez-AdánP Lonergan
Jan 15, 2005·Antioxidants & Redox Signaling·Samuel SigaudBeatriz González-Flecha
Jan 27, 2005·Cloning and Stem Cells·Ian Chambers
May 7, 2005·Contributions to Nephrology·Francesco GalliArdesio Floridi
May 18, 2005·Neurochemical Research·Won Chang LeeYong Keun Kim-
May 24, 2005·Reproductive Toxicology·Mellisa A HausburgCatherine S Gardiner
Jul 16, 2005·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalRakesh K Sharma
Aug 19, 2005·American Journal of Physiology. Cell Physiology·Jung Sun HeoHo Jae Han

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Citations

Jan 17, 2013·Expert Opinion on Biological Therapy·Hayat MuhammadNicolai Miosge
Nov 3, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Jae Chun LimUy Dong Sohn
Jul 28, 2011·Experimental Animals·Shuji Takabayashi, Hideki Katoh
Sep 30, 2017·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S-Y LinC H Kao

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Methods Mentioned

BETA
electrophoresis

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