Effect of dihydrotestosterone on the growth and function of ovarian follicles in intact immature female rats primed with PMSG

Journal of Reproduction and Fertility
B A ConwayT M Mills

Abstract

Intact, immature female rats were primed with PMSG and treated with 4 injections of DHT. DHT given at 0, 12, 24 and 36 h caused a significant decrease in the ovulation rate 72 h after the PMSG treatment. Concurrent treatment with oestrogen reversed the inhibitory effects of the androgen. The androgen effect was apparently exerted directly on the ovary since DHT did not alter the surge of LH and FSH which occurred at 58 h after PMSG treatment. The DHT inhibition of ovulation was observed in the treatment cycle as well as in subsequent cycles which followed a second PMSG injection. This finding suggests that intermediate size follicles were also adversely affected by the androgen. To confirm that androgen affects follicles of all size ranges, follicles less than 200 microns, 200-400 microns and greater than 400 microns in diameter were isolated from the ovaries of rats treated with PMSG and DHT or the vehicle. The follicles were isolated by density gradient separation of follicles followed by filtration with pre-calibrated Teflon sieves. In some experiments, granulosa cells were also harvested from isolated follicles. DHT treatment did not affect the numbers of follicles of any size but did reduce the oestrogen content of follicl...Continue Reading

Citations

Sep 2, 1998·Molecular and Cellular Endocrinology·M TetsukaS G Hillier
Oct 27, 2011·American Journal of Physiology. Endocrinology and Metabolism·Virendra B Mahesh
Apr 11, 2006·Reproductive Biology and Endocrinology : RB&E·Ann E Drummond
Mar 11, 2015·The Journal of Reproduction and Development·Miho Makita, Takashi Miyano
Apr 20, 2007·Trends in Endocrinology and Metabolism : TEM·Shuhei KimuraShigeaki Kato
Apr 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·M Tetsuka, S G Hillier
Sep 12, 2006·Domestic Animal Endocrinology·Masa Tetsuka, Colin D Nancarrow
Dec 18, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·K A Walters
Aug 16, 2008·Biology of Reproduction·Esbal JiménezWilliam F Pope
Feb 16, 2012·Endocrinology·E Leonie A F van HoutenJenny A Visser
Mar 5, 2019·Reproduction, Fertility, and Development·Yishu WangChunping Zhang

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