Effect of dilution rate on lipopolysaccharide and serum resistance of Neisseria gonorrhoeae grown in continuous culture.

Infection and Immunity
S A MorseM A Apicella

Abstract

Growth of Neisseria gonorrhoeae strain FA171 in continuous culture under glucose-limiting conditions resulted in a growth-rate-dependent change in the lipopolysaccharide (LPS). The evidence for this change is an alteration in the mobility of purified alkali-treated LPS on sodium dodecyl sulfate-polyacrylamide gels and a quantitative difference in the amount of the LPS serotype antigen. The LPS from cells grown at a low dilution rate (0.12 h-1) contained ca. eightfold less serotype antigen than the LPS from cells grown at a high dilution rate (0.56 h-1). The decrease in LPS serotype antigen was associated with an increase in sensitivity to the bactericidal activity of normal human serum and an increase in cell surface hydrophobicity. An increase in the amount of serotype antigen was associated with a reduction in the accessibility of a monoclonal antibody to a core LPS determinant, an increase in resistance to normal human serum, and a decrease in cell surface hydrophobicity. The microheterogeneity of gonococcal LPS with respect to the content of serotype antigen may result from an alteration in the metabolism of glucose.

References

Jul 1, 1978·Infection and Immunity·S A Morse, B H Hebeler
Jan 1, 1978·CRC Critical Reviews in Microbiology·S A Morse
May 1, 1975·Canadian Journal of Biochemistry·M B Perry, V Daoust
Jan 1, 1978·Annual Review of Microbiology·C J van Oss
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Nov 1, 1974·Journal of Bacteriology·S A MorseJ Hines
Apr 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·S A Morse, L Bartenstein
Feb 1, 1971·Journal of General Microbiology·I J McDonald, G A Adams
Apr 1, 1980·Canadian Journal of Microbiology·P Z AllenM A Apicella
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jan 1, 1980·Canadian Journal of Microbiology·S A Morse, L Bartenstein
May 1, 1981·Infection and Immunity·J G CannonP F Sparling
Jan 1, 1982·The Journal of Infectious Diseases·J F JamesG F Brooks
May 1, 1982·The Journal of Infectious Diseases·A I Braude

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Citations

Jun 27, 1985·The New England Journal of Medicine·B E BritiganP F Sparling
Oct 1, 1984·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·E Rødahl, J A Maeland
Jun 1, 1995·Molecular Microbiology·J P van Putten, B D Robertson
Nov 12, 2002·Journal of Bacteriology·Andrzej Piekarowicz, Daniel C Stein
Jan 1, 1996·Critical Reviews in Microbiology·A PrestonM A Apicella
Oct 24, 2008·Biotechnology and Bioengineering·Gino J E BaartDirk E Martens
Jan 15, 1999·Critical Reviews in Microbiology·C M Kahler, D S Stephens
Sep 1, 1996·Progress in Lipid Research·S G Wilkinson
Nov 1, 1991·Antimicrobial Agents and Chemotherapy·J C HurleyJ B Carlin
Oct 1, 1986·Infection and Immunity·R MandrellJ M Griffiss
Dec 1, 1986·Infection and Immunity·H SchneiderJ M Griffiss
Apr 1, 1989·Clinical Microbiology Reviews·P A Rice

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