Effect of dimethylsulfoxide and hydroxyethyl starch in the preservation of fractionated human marrow cells

Cryobiology
K H LuoH Liu

Abstract

Fractionated human bone marrow cells, isolated by density centrifugation, could be well cryopreserved in both a DMSO/HES/albumin mixture and the conventional cryoprotectant (DMSO/serum) with either a programmed freezer or a -80 degrees C freezer. A lower initial temperature rise and a delayed plateau phase in the mixture in comparison with those in the DMSO/serum were observed using a programmed procedure without heat fusion compensation. Plateau phase in the mixture could also be shortened with increased liquid nitrogen to minimize the transition phase duration. However, the method of mononuclear cells immersed in a -80 degrees C freezer and stored in liquid nitrogen is simple and easily standardized. Additionally, widely used lactated Ringer's solution instead of Normosol-R can be employed to prepare the mixture for the preservation of fractionated cells.

Citations

Feb 22, 2012·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·A StolzingS Sethe
Oct 7, 2016·Scientific Reports·A Billal SultaniLocksley E McGann
Aug 16, 2017·Oxidative Medicine and Cellular Longevity·Abhishek Kumar SinghAkalabya Bissoyi

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