Effect of dissociative methods on cortisol binding and glutamyltransferase inducibility in chick embryo retina

Cell Biophysics
J Piperberg, L Reif-Lehrer

Abstract

The ability of the isolated embryonic chick retina (12 days) to bind a steroid (cortisol) decreases when the tissue is dissociated; the extent of this decrease depends upon the method of dissociation. Trypsin and mechanical dissociation decreased cortisol binding slightly; papain dissociation essentially eliminated it. Cortisol binding decreased with time in culture in both whole retina and monolayer cultures; this decrease may reflect, in part, a similar development decrease in ovo. Inducibility of glutamine synthetase in whole retinas and retinal monolayers prepared with either trypsin or papain also decreased with time in culture. For whole and trypsin-dissociated retinas, the drop in inducibility correlates with the drop in cortisol-binding capacity. This was not the case for monolayer cultures prepared by papain dissociation.

References

Feb 1, 1975·Experimental Eye Research·L Reif-Lehrer, D Lindewall
Sep 1, 1975·Archives of Biochemistry and Biophysics·D E Koehler, A A Moscona
Jan 1, 1979·Monographs on Endocrinology·G G Rousseau, J D Baxter
Feb 1, 1978·Molecular and Cellular Endocrinology·P K Sarkar, J L Lydigsen
Mar 17, 1973·Biochemical and Biophysical Research Communications·R E JonesA A Moscona
Nov 25, 1973·Experimental Eye Research·L Reif-Lehrer, J Coghlin
Jun 4, 1971·Biochemical and Biophysical Research Communications·G J Chader
Feb 15, 1974·Science·B W O'Malley, A R Means
May 1, 1974·Experimental Eye Research·B O Wiggert, G J Chader
May 24, 1972·Nature: New Biology·R J Schwartz
Mar 24, 1972·Biochemical and Biophysical Research Communications·G J ChaderJ Silver
Mar 1, 1971·Archives of Ophthalmology·G J ChaderL Reif-Lehrer
Apr 6, 1966·Biochemical and Biophysical Research Communications·L Reif, H Amos
Mar 1, 1981·Cell Biophysics·K Dutt, L Reif-Lehrer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.