Effect of dissolved organic nitrogen contamination on δ15 N-NH4 determination in water samples by modification of the diffusion method with gas-phase trapping

Rapid Communications in Mass Spectrometry : RCM
Thi Thuy Hai CaoKei Nishida

Abstract

The isotopic tracer technique is widely used to identify the sources and fate of nitrogen (N) in order to understand the N cycle and contamination in water environments. The stable isotope ratio of ammonium is expected to greatly enhance the tracing analysis by combining it with the traditional nitrate isotope ratio. Diffusion followed by gas-phase trapping is the most commonly applied method for ammonium isotope ratio measurement. Although dissolved organic nitrogen (DON) is abundant in natural water and its breakdown in the diffusion procedure has been reported, the interference of DON with the measurement of ammonium isotope ratios has not been fully examined. This study aims to test the effect of DON contamination by using organic N compounds, viz. humic acid and alanine. A series of diffusion experiments was conducted at a temperature of 80°C for a maximum of 7 days. Ammonia was transferred from alkaline solution and trapped with an acidic filter. This method was applied for samples with ammonium concentrations between 0.5 and 2.0 mg-N/L. There was no difference between the ammonium N stable isotope ratios for samples with and without added DON compounds; the fractionation between the observed value and the actual value wa...Continue Reading

References

May 14, 2009·The Science of the Total Environment·Yu UmezawaMakoto Taniguchi
Mar 25, 2014·Analytical Chemistry·Dongwei LiuYuepeng Pan

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