Abstract
Highly efficient and stable enzymes are required for application in biotechnology, to meet the technical, environmental, and economic industrial demands. Xylanases are hemicellulolytic enzymes that degrade the heteroxylan constituent of the lignocellulosic plant cell wall. In this study, an acidic xylanase designated Pjxyn (pH 4.0) from Penicillium janthinellum was engineered by the introduction of a disulfide bridge. This strategy exploited the influence of the bridge on hydrolysis characteristics and enhanced hydrolysis was achieved. Three mutants [PjxynS(27)S(39), PjxynS(27)S(186), and PjxynS(39)S(186)] produced more xylose and xylobiose as hydrolysis products compared with the wild-type Pjxyn, when commercial xylans and lab-prepared water-insoluble corncob-xylan were used as the substrates, especial for the PjxynS(27)S(39) mutant, the content of xylose and xylobiose was 87.62% (using beechwood xylan) and 69.91% (using oat-spelt xylan) higher than that in the hydrolysis products of Pjxyn. Moreover, each mutant combined with the xylanase mutant T-XynFM effectively decreased the production of xylose with an optimum xylobiose yield. The findings demonstrate the potential industrial value of engineering xylanase to improve its h...Continue Reading
References
Nov 1, 1994·Protein Engineering·W W WakarchukM Yaguchi
Dec 1, 1994·Current Opinion in Structural Biology·J D McCarter, S G Withers
Sep 30, 1998·Biochemistry·K GruberC Kratky
Jul 28, 1999·FEMS Microbiology Reviews·N KulkarniM Rao
Sep 3, 1999·The Journal of Biological Chemistry·J S KimB H Oh
Jan 7, 2000·Journal of Molecular Biology·P R KumarM A Viswamitra
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·C Vieille, G J Zeikus
Mar 26, 2002·Journal of Biotechnology·Amalia SapagEric Depiereux
Mar 26, 2003·European Journal of Biochemistry·Nina HakulinenJuha Rouvinen
Oct 11, 2003·Biotechnology Advances·M K Bhat
May 8, 2004·Journal of Biotechnology·Fred FenelOssi Turunen
Nov 13, 2004·Journal of Agricultural and Food Chemistry·Johnny BeaugrandBrigitte Chabbert
Jan 18, 2005·FEMS Microbiology Reviews·Tony CollinsGeorges Feller
Jun 18, 2005·Letters in Applied Microbiology·Z Q JiangX T Li
Feb 14, 2006·Journal of Bioscience and Bioengineering·Hidenori TanakaKazuyoshi Ohta
Apr 29, 2006·Journal of Biotechnology·Gabriel Paës, Michael J O'Donohue
Dec 2, 2006·Journal of Industrial Microbiology & Biotechnology·H M YangN F Wu
Aug 19, 2007·British Poultry Science·F GaoZ K Han
Mar 6, 2008·Biotechnology Letters·Jean-Guy Berrin, Nathalie Juge
Jun 9, 2009·Current Opinion in Biotechnology·Antoine MargeotFrédéric Monot
Sep 9, 2009·Bioresource Technology·Min ZhangLite Li
Nov 10, 2011·Biotechnology Advances·Gabriel PaësJohnny Beaugrand
Mar 20, 2012·Bioresource Technology·Yawei WangOssi Turunen
Apr 19, 2012·Journal of Food Science·Yunping ZhuHongxia Song
Jul 12, 2012·Biotechnology Letters·Guangsen FanZhengqiang Jiang
Oct 9, 2013·Journal of Industrial Microbiology & Biotechnology·Digvijay Verma T Satyanarayana
Dec 26, 2016·International Journal of Biological Macromolecules·Qin LiXiuting Li
Mar 31, 2017·International Journal of Biological Macromolecules·Qin LiXiuting Li
Oct 17, 2017·International Journal of Biological Macromolecules·Qin LiChao Teng