Effect of diverse mammalian gonadotropins on estrogen and progesterone production by cultured rat granulosa cells

Archives of Biochemistry and Biophysics
A J HsuehH Papkoff

Abstract

The ability of gonadotropins from six mammalian species to stimulate estrogen and progesterone production was investigated in granulosa cells of hypophysectomized estrogen-primed immature female rats. Granulosa cells were cultured for 2 days in the presence of delta 4-androstenedione (10(-7) M) with or without various gonadotropin preparations. Treatment with follitropin (follicle-stimulating hormone, FSH) from human, rat, ovine, porcine, equine, and bovine origins resulted in dose-dependent increases in steroidogenesis from negligible amounts to maximal levels of approximately 4-8 and 12-30 ng/10(5) cells for estrogen and progesterone, respectively. The ED50 values of the FSH preparations for stimulation of steroidogenesis were: human: 1-4 ng/ml; ovine: 2.5-30 ng/ml; rat: 1.6-4.0 ng/ml; porcine: 7.5-20 ng/ml; equine 2.5-6 ng/ml; and bovine greater than 100 ng/ml. Lutropin (luteinizing hormone, LH) from rat, ovine, bovine, and porcine origins, human chorionic gonadotropin (hCG), the alpha-subunit of human FSH and the beta-subunit of human LH were ineffective in stimulating steroidogenesis, indicating the specificity of the assay system for FSH. In a high concentration (600 ng/ml), the beta-subunit of human FSH-stimulated steroi...Continue Reading

References

Nov 1, 1976·Endocrinology·D T Armstrong, J H Dorrington
Mar 15, 1972·Biochimica Et Biophysica Acta·D Schams, H Papkoff
Aug 1, 1961·Proceedings of the Society for Experimental Biology and Medicine·B D SMITH, J T BRADBURY

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Citations

Aug 1, 1984·Molecular and Cellular Endocrinology·T H WelshA J Hsueh
Jan 1, 1988·Molecular and Cellular Endocrinology·L A HutchinsonA C Herington
Dec 2, 1996·Animal Reproduction Science·M KuranP J Broadbent
Aug 1, 1990·General and Comparative Endocrinology·J R McFarlaneH Papkoff
Mar 4, 2000·Endocrine Reviews·M P RoseA H Balen
Dec 1, 1991·Journal of Endocrinological Investigation·M Simoni, E Nieschlag
Feb 15, 2021·Cell and Tissue Research·Myung Jae JeonJohn D Jackson

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