Effect of diverse tumor promoters on the expression of gap-junctional proteins connexin (Cx)26, Cx31.1, and Cx43 in SENCAR mouse epidermis

Molecular Carcinogenesis
I V BudunovaT J Slaga

Abstract

The inhibition of gap-junctional intercellular communication (GJIC) between initiated and surrounding normal cells by tumor promoters is believed to be important in the promotion stage of carcinogenesis. Therefore, we examined the effect of skin-tumor promoters on the expression of the gap-junctional proteins connexin (Cx) 26, Cx43, and Cx31.1 in SENCAR mouse skin. Animals were treated with 12-0-tetradecanoylphorbol-13-acetate (TPA) (8.3 nmol), okadaic acid (OA) (2.5 nmol), chrysarobin (220 nmol), or benzoyl peroxide (BzPo) (83 micromol). Northern blot and immunofluorescence analyses revealed that keratinocytes in adult mouse skin expressed Cx31.1 and Cx43 but not Cx26. All four of the skin-tumor promoters switched on the Cx26 gene, transiently increased expression of Cx43, and significantly inhibited the expression of Cx31.1. The time courses for changes in Cx26, Cx3l. 1, and Cx43 mRNA levels coincided in most cases and in general corresponded well to the time-response curves for hyperplastic changes in mouse skin. The peaks of Cx26 and Cx43 expression and Cx31.1 inhibition appeared 12 h after TPA application and 24 h after OA and chrysarobin application. BzPo elevated the levels of Cx26 and Cx43 transcripts later (peak at 2-4...Continue Reading

References

Feb 4, 1979·Biochimica Et Biophysica Acta·W R Loewenstein
Nov 28, 1979·Biochemical and Biophysical Research Communications·A W Murray, D J Fitzgerald
May 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M Karin
Aug 31, 1992·Biochemical and Biophysical Research Communications·P RieckmannJ H Kehrl
Jan 11, 1992·Nucleic Acids Research·S Faisst, S Meyer
Mar 1, 1991·Neuron·M V BennettJ C Sáez
Jan 1, 1988·Molecular Carcinogenesis·M MesnilH Yamasaki
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M SuganumaT Sugimura
Jun 1, 1985·The Journal of Investigative Dermatology·G K MenonP M Elias
Jul 24, 1982·Nucleic Acids Research·A BalmainG D Birnie
Jul 1, 1982·The Journal of Investigative Dermatology·A J Klein-Szanto, T J Slaga
Apr 1, 1995·Molecular Biology of the Cell·T W WhiteR Bruzzone
Mar 1, 1994·Journal of Molecular and Cellular Cardiology·J R De LeonG I Fishman
May 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J A Goliger, D L Paul
Mar 3, 1994·Nature·M V Bennett
Feb 22, 1994·Proceedings. Biological Sciences·W YuR Werner

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Citations

Dec 31, 2002·Toxicology·Edmond Ekué CreppyMaria-Rosaria Carratú
Jul 6, 2001·The Biochemical Journal·L ShoreM E Finbow
Sep 3, 2005·Biochimica Et Biophysica Acta·Timothy J King, Paul D Lampe
Feb 16, 1999·Journal of Surgical Oncology·R P HuangA L Boynton
Feb 1, 2005·European Journal of Cell Biology·Markus KretzKlaus Willecke
Aug 11, 2005·Molecular Cancer·Trond AasenMalcolm B Hodgins

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