Effect of DNA methylation on protein-DNA interaction of HL-60 cells

Science in China. Series C, Life Sciences
Z HeY Zhang

Abstract

HL-60 cells have been induced with differentiation index 16% by S-adenosyl-L-methionine (SAM) as inducer in the presence of optimum concentration of 10 pmol/L. The methylation level of genome DNA determined by HPLC is increased during cell differentiation. When restriction endonucleaseHae III,Sma I,Sal I,XhoI andHind III which are sensitive to 5-methyicytoside were used to cleave the genome DNA, a resistance effect was found. The interaction between DNA and DNA binding proteins is changed by using gel retarding test.

References

Jul 22, 1991·FEBS Letters·J Lewis, A Bird
Jan 1, 1991·Molecular Carcinogenesis·S S Smith

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