Effect of dodecylmaltoside (DDM) on uptake of BCS III compounds, tiludronate and cromolyn, in Caco-2 cells and rat intestine model

Drug Delivery
Deepali D DeshmukhGuru V Betageri

Abstract

The efficacy of n-lauryl-beta-D-maltopyranoside, (dodecylmaltoside, DDM) as a permeability-enhancer for tiludronate and cromolyn (BCS Class III, water-soluble compounds with oral bioavailability < 5%) was evaluated in Caco-2 cell monolayers and rat intestinal sacs. In Caco-2 cells samples were collected over a 5-h period and transepithelial resistance (TEER) was measured concurrently. In rat intestinal sacs, samples of the test compounds and marker (Lucifer Yellow) were collected over a 40 min period; accumulation in the serosal fluid and intestinal tissue was measured. At lower concentration DDM had no effect on cromolyn permeability and a marginal increase was observed at higher concentration. Tiludronate permeability in the presence of DDM showed greater enhancement as compared to cromolyn. At higher concentration DDM appeared to cause permanent damage to the cell monolayer (irreversible change in TEER). In the intestinal tissue, DDM caused increased tissue accumulation of test compounds. This indicated that transport was not restricted to the paracellular route and damage to the intestinal tissue could not be ruled out. Based on the results obtained in this study it can be concluded that at concentrations that are non-toxic...Continue Reading

References

Apr 1, 1990·European Journal of Drug Metabolism and Pharmacokinetics·G Wilson
Sep 1, 1990·Pharmaceutical Research·A R HilgersP S Burton
Feb 1, 1984·The Journal of Nutrition·N Weber, H Benning
Feb 15, 1983·Biochemical Pharmacology·P G Ruifrok, W E Mol
Apr 1, 1981·Physiology & Behavior·A Poplawsky, J S McVeigh
Jul 1, 1994·Journal of Pharmaceutical Sciences·P S Leppert, J A Fix
Feb 1, 1996·Pharmaceutical Research·A Leone-BayR A Baughman
Sep 2, 1998·European Journal of Drug Metabolism and Pharmacokinetics·L BartheG Houin
Jun 24, 1999·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·C Meaney, C O'Driscoll
Nov 26, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·H DaviJ F Thiercelin
Dec 1, 1962·Proceedings of the Society for Experimental Biology and Medicine·C S TIDBALL, R I LIPMAN
Dec 1, 2004·Journal of Pharmaceutical Sciences·Phani Prasanth Tirumalasetty, John G Eley

❮ Previous
Next ❯

Citations

Jan 14, 2012·Drug Metabolism and Pharmacokinetics·Jin Sook SongMyung Ae Bae
Jan 7, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Cong LuoZhonggui He
Jun 21, 2016·Advanced Drug Delivery Reviews·Sam MaherDavid J Brayden

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Related Papers

Critical Reviews in Therapeutic Drug Carrier Systems
Sumit KumarSaahil Arora
European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
Michael LinnClaus-Michael Lehr
Expert Opinion on Drug Metabolism & Toxicology
Teruo Murakami, Mikihisa Takano
Journal of Pharmacological and Toxicological Methods
C A Lipinski
© 2021 Meta ULC. All rights reserved