Effect of donor age on the proportion of mesenchymal stem cells derived from anterior cruciate ligaments

PloS One
Dae-Hee LeeSeung-Beom Han

Abstract

The characteristics of anterior cruciate ligament (ACL)-derived mesenchymal stem cells (MSCs), such as proportion and multilineage potential, can be affected by donor age. However, the qualitative and quantitative features of ACL MSCs isolated from younger and older individuals have not yet been compared directly. This study assessed the phenotypic and functional differences in ACL-MSCs isolated from younger and older donors and evaluated the correlation between ACL-MSC proportion and donor age. Torn ACL remnants were harvested from 36 patients undergoing ACL reconstruction (young: 29.67 ± 10.92 years) and 33 undergoing TKA (old: 67.96 ± 5.22 years) and the proportion of their MSCs were measured. The mean proportion of MSCs was slightly higher in older ACL samples of the TKA group than of the younger ACL reconstruction group (19.69 ± 8.57% vs. 15.33 ± 7.49%, p = 0.024), but the proportions of MSCs at passages 1 and 2 were similar. MSCs from both groups possessed comparable multilineage potentiality, as they could be differentiated into adipocytes, osteocytes, and chondrocytes at similar level. No significant correlations were observed between patient age and MSC proportions at passages 0-2 or between age and MSC proportion in b...Continue Reading

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Citations

May 24, 2016·Stem Cells International·Kulraj Singh BhangraAhad A Rahim
Dec 19, 2016·European Journal of Cell Biology·Sajjad SisakhtnezhadHassan Akrami
Nov 6, 2018·Journal of Cellular Biochemistry·Biao ChenJames H-C Wang
Oct 8, 2019·Stem Cells and Development·Takashi TaguchiDominique J Griffon
Apr 8, 2017·Stem Cells International·Katie Joanna LeeElizabeth Gail Canty-Laird
Jan 23, 2019·Journal of Proteome Research·Katie J LeeElizabeth G Canty-Laird
Oct 5, 2021·Frontiers in Cell and Developmental Biology·Wasco WruckJames Adjaye

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Datasets Mentioned

BETA
GSE59662

Methods Mentioned

BETA
FACS
flow cytometry

Software Mentioned

R
Illumina GenomeStudio

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