Effect of dose rate on residual γ-H2AX levels and frequency of micronuclei in X-irradiated mouse lymphocytes

Radiation Research
H C TurnerD J Brenner

Abstract

The biological risks associated with low-dose-rate (LDR) radiation exposures are not yet well defined. To assess the risk related to DNA damage, we compared the yields of two established biodosimetry end points, γ-H2AX and micronuclei (MNi), in peripheral mouse blood lymphocytes after prolonged in vivo exposure to LDR X rays (0.31 cGy/min) vs. acute high-dose-rate (HDR) exposure (1.03 Gy/min). C57BL/6 mice were total-body irradiated with 320 kVP X rays with doses of 0, 1.1, 2.2 and 4.45 Gy. Residual levels of total γ-H2AX fluorescence in lymphocytes isolated 24 h after the start of irradiation were assessed using indirect immunofluorescence methods. The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used to determine apoptotic cell frequency in lymphocytes sampled at 24 h. Curve fitting analysis suggested that the dose response for γ-H2AX yields after acute exposures could be described by a linear dependence. In contrast, a linear-quadratic dose-response shape was more appropriate for LDR exposure (perhaps reflecting differences in repair time after different LDR doses). Dose-rate sparing effects (P < 0.05) were observed at doses ≤2.2 Gy, such that the acute dose γ-H2AX and TUNEL-positive cell yi...Continue Reading

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Citations

Jun 27, 2015·Radiation Research·Sunirmal PaulSally A Amundson
Aug 24, 2019·Radiation Oncology·Younghyun LeeHelen C Turner
May 2, 2020·The British Journal of Radiology·Daisuke KawaharaYasushi Nagata
Sep 1, 2017·Environmental and Molecular Mutagenesis·Anne GraupnerAnn Karin Olsen
Feb 18, 2017·Radiation Research·Guy GartyDavid J Brenner
Jan 4, 2020·Radiation and Environmental Biophysics·Qi WangHelen C Turner
Dec 24, 2018·International Journal of Molecular Sciences·Yuanyuan MengWenqing Xu
Jul 20, 2019·Scientific Reports·Stephen G R BarnardElizabeth Ainsbury
Oct 31, 2019·International Journal of Molecular Sciences·Shankari NairCharlot Vandevoorde
May 19, 2021·Scientific Reports·Jian GuFrederic Zenhausern

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Methods Mentioned

BETA
chromosomal aberrations
electrophoresis
biopsies
X-Ray
blood collection
Assay
X Rays

Software Mentioned

Excel

Related Concepts

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