PMID: 9188283Jun 1, 1997Paper

Effect of dosimetric and physiological factors on the lethal photosensitization of Porphyromonas gingivalis in vitro

Photochemistry and Photobiology
M BhattiM Wilson

Abstract

The aims of this study were to (1) determine the effect of dosimetric and physiological factors on the lethal photosensitization of Porphyromonas gingivalis using toluidine blue O (TBO) and light from a helium/neon (HeNe) laser; (2) determine the influence of sensitizer concentration, preirradiation time, serum and growth phase on sensitizer uptake by P. gingivalis. The dosimetric factors studied were concentration of TBO, light dose and preirradiation time. The physiological factors were presence of serum, pH and bacterial growth phase. Sensitizer uptake by P. gingivalis under various conditions was determined using tritiated TBO (3H-TBO). In the presence of TBO, a light dose-dependent increase in kill was attained (100% kill at 4.4 J). There was no significant effect on the numbers killed when TBO was increased from 12.5 to 50 micrograms/mL. An increase in preirradiation time gave slightly increased kills. High kills were achieved at all three pH (6.8-8.0). Although kills were substantial in the presence of serum, they were significantly less than those obtained in the presence of saline. Cells in all three growth phases were susceptible to lethal photosensitization, although stationary phase cells were slightly less suscepti...Continue Reading

References

Jul 1, 1975·Photochemistry and Photobiology·R PottierJ Joussot-Dubien
Nov 1, 1992·Archives of Oral Biology·J Dobson, M Wilson
Aug 1, 1992·Current Microbiology·M WilsonW Harvey
May 1, 1990·Journal of Photochemistry and Photobiology. B, Biology·Z MalikY Nitzan
Jan 1, 1985·The Journal of Infectious Diseases·F R VenezioO H Reichman
Jul 1, 1966·Photochemistry and Photobiology·J D MacmillanC O Chichester
Mar 1, 1994·The Journal of Antimicrobial Chemotherapy·M Wilson, J Pratten
Jun 1, 1993·Oral Microbiology and Immunology·M WilsonS Sarkar
Jun 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M Wilson, J Dobson
Apr 1, 1995·Anaerobe·J A García-RodríguezJ L Muñoz-Bellido

❮ Previous
Next ❯

Citations

Feb 22, 2002·Lasers in Surgery and Medicine·Selçuk YilmazTanju Kadir
Oct 2, 2009·Current Microbiology·Sarit Oriel, Yeshayahu Nitzan
Sep 28, 2012·Clinical Oral Investigations·Hare GursoySelçuk Yilmaz
Mar 16, 2007·Lasers in Medical Science·Yanli QinZhiguo Zhang
Jul 7, 2009·Lasers in Medical Science·J LinT T Dong
May 10, 2005·Journal of Photochemistry and Photobiology. B, Biology·Peter Meisel, Thomas Kocher
May 22, 1998·Journal of Photochemistry and Photobiology. B, Biology·Y NitzanH Ashkenazi
Aug 12, 1999·Journal of Photochemistry and Photobiology. B, Biology·S WoodC Robinson
Jul 17, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Michelle L EmbletonMichael Wilson
Dec 27, 2007·Photomedicine and Laser Surgery·I M BevilacquaM T T Pacheco
Jun 15, 2012·Photomedicine and Laser Surgery·Natália Costa AraújoMarleny Elizabeth Martinez Gerbi
Oct 22, 2013·Photomedicine and Laser Surgery·Fawad Javed, Georgios E Romanos
Sep 19, 2000·Antimicrobial Agents and Chemotherapy·M BhattiM Wilson
May 25, 2004·Antimicrobial Agents and Chemotherapy·Faten GadMichael R Hamblin
Mar 6, 2007·Journal of Periodontology·Juliano Milanezi de AlmeidaValdir Gouveia Garcia
Jun 7, 2008·Journal of Periodontology·Juliano Milanezi de AlmeidaValdir Gouveia Garcia
Nov 5, 2008·Journal of Periodontology·Juliano Milanezi de AlmeidaValdir Gouveia Garcia
Nov 13, 2009·Journal of Periodontology·Pam BrahamRichard Darveau
Apr 25, 2012·Lasers in Medical Science·Valdir Gouveia GarciaLetícia Helena Theodoro
Apr 23, 2008·Lasers in Medical Science·Sérgio Luiz PinheiroMariângela Cagnori Ribeiro
May 12, 2009·Lasers in Medical Science·Sérgio Luiz PinheiroPatrícia Fernanda Roesler Bertolini
Feb 27, 2015·Pharmaceutical Development and Technology·Milica Vukićević, Hanne Hjorth Tønnesen
Oct 17, 2015·International Journal of Molecular Sciences·Takeshi KikuchiAkio Mitani
Feb 15, 2001·Expert Opinion on Investigational Drugs·I StojiljkovicV Kumar
Nov 10, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Annie ShresthaAnil Kishen
Feb 19, 2009·Journal of Photochemistry and Photobiology. B, Biology·Corona M CassidyRyan F Donnelly
Oct 27, 2015·Photodiagnosis and Photodynamic Therapy·Zuzanna OrubaMaria Chomyszyn-Gajewska
May 24, 2008·Journal of Endodontics·Jacob Lee FimpleNikolaos S Soukos
Jan 2, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ryan F DonnellyMichael M Tunney
Sep 18, 2009·European Journal of Oral Sciences·Juliana P M LimaIriana C J Zanin
Nov 3, 2009·Periodontology 2000·Aristeo A TakasakiYuichi Izumi
Sep 10, 2009·Australian Dental Journal·M RaghavendraS Bhola
May 5, 2006·Clinical Oral Implants Research·Jamil Awad ShibliElcio Marcantonio
Dec 8, 2010·Periodontology 2000·Nikolaos S Soukos, J Max Goodson
Jul 16, 2009·Journal of Periodontal Research·C R FontanaN S Soukos
Dec 17, 2014·Periodontology 2000·Euloir PassaneziSebastião L Aguiar Greghi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Michael R Hamblin, Tayyaba Hasan
Journal of the National Cancer Institute
T J DoughertyQ Peng
© 2021 Meta ULC. All rights reserved