Effect of downregulation of ZEB1 on vimentin expression, tumour migration and tumourigenicity of melanoma B16F10 cells and CSCs

Cell Biology International
Jun DouJing Wang

Abstract

Zinc-finger E-box binding homeobox 1 (ZEB1) is a master regulator of epithelial-mesenchymal transition (EMT) and has been implicated in primary epithelial cancer biological processes, such as invasion and metastasis. However, the role of ZEB1 in progression of melanoma and cancer stem cells (CSCs) remains obscure. In this study, the recombinant plasmids of t3 shRNAs targeting mouse ZEB1 were constructed and transfected into melanoma B16F10 cells. The stable transfected cells were selected and the characteristics of ZEB1 downregulated B16F10 cells was assessed. The tumourigenicity of CD44(+) CD133(+) CSCs isolated from B16F10 cells stably transfected with the ZEB1-shRNA2 recombinant was also assessed. ZEB1-shRNAs B16F10 showed a lower expression of ZEB1 and vimentin, weaker migration, invasiveness, colony forming, and proliferation, and a lower tumourigenicity than the control cells. The tumourigenicity of the ZEB1-shRNA2 CD44(+) CD133(+) CSCs was also inhibited. In conclusion, ZEB1-shRNA2-mediated downregulation of ZEB1 expression in B16F10 cells and CSCs is involved in the inhibition of the EMT process. ZEB1 may be a potential target in melanoma targeted.

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Citations

Mar 13, 2014·Sensors·Clotilde CostaLaura Muinelo-Romay
Nov 13, 2015·Bioscience Trends·Fengshu ZhaoJun Dou
May 3, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaoying WangJun Dou
Apr 30, 2015·Japanese Journal of Clinical Oncology·Xianglun SunJie Zhang
Dec 30, 2014·Frontiers in Oncology·Niels Vandamme, Geert Berx
Nov 5, 2016·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Hongyan ZangJingguang Hao
Jul 13, 2016·Journal of Cellular Physiology·Federico Castro-MuñozledoErika Sánchez-Guzmán
Oct 25, 2017·Cancer Biomarkers : Section a of Disease Markers·Yanni LiuYing Wang
Sep 7, 2016·EMBO Molecular Medicine·Geoffrey RichardJulie Caramel

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