Effect of dual treatment with SDF-1 and BMP-2 on ectopic and orthotopic bone formation

PloS One
Chang-Hwan LeeTae-Geon Kwon

Abstract

The potent stem cell homing factor stromal cell-derived factor-1 (SDF-1) actively recruits mesenchymal stem cells from circulation and from local bone marrow. It is well established that bone morphogenetic protein-2 (BMP-2) induces ectopic and orthotopic bone formation. However, the exact synergistic effects of BMP-2 and SDF-1 in ectopic and orthotopic bone regeneration models have not been fully investigated. The purpose of this study was to evaluate the potential effects of simultaneous SDF-1 and BMP-2 treatment on bone formation. Various doses of SDF-1 were loaded onto collagen sponges with or without BMP-2.These sponges were implanted into subcutaneous pockets and critical-size calvarial defects in C57BL/6 mice. The specimens were harvested 4 weeks post-surgery and the degree of bone formation in specimens was evaluated by histomorphometric and radiographic density analyses. Osteogenic potential and migration capacity of mesenchymal cells and capillary tube formation of endothelial cells following dual treatment with SDF-1 and BMP-2 were evaluated with in vitro assays. SDF-1-only-treated implants did not yield significant in vivo bone formation and SDF-1 treatment did not enhance BMP-2-induced ectopic and orthotopic bone re...Continue Reading

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Citations

Nov 22, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ya-Min LiJin-Zhong Zhao
Sep 3, 2020·Tissue Engineering. Part B, Reviews·Jeremie D OliverRena N D'Souza
Jul 4, 2017·Journal of Biological Engineering·Wen ZhaoSong Li
Dec 2, 2017·Drug Delivery and Translational Research·Mathieu MaisaniOlivier Chassande
Oct 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dapeng ZhaoHemin Nie

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