Effect of early acute high concentrations of iodide exposure on mitochondrial superoxide production in FRTL cells

Free Radical Biology & Medicine
Xiaomei YaoLanying Li

Abstract

Excessive oxidative stress has been suggested as one of the underlying mechanisms in the development of thyroid cytotoxicity. Although the involvement of mitochondria has been hypothesized, the effect of early acute high concentrations of iodide on mitochondrial superoxide production remains largely unknown, especially within a 24 h time frame. By using a novel fluorescent probe, MitoSOX Red, we demonstrated the concentration response and time-course response of KI-induced mitochondrial superoxide production in the Fischer rat thyroid cell line (FRTL). A strong increase of MitoSOX Red fluorescence intensity in FRTL cells can be seen at 2 h following high concentrations of iodide exposure. Besides, we indicated that 6-propyl-2-thiouracil (PTU, 300 μM), thyroid-stimulating hormone (TSH, 10 mU/ml), and perchlorate (KClO(4), 30 μM) can inhibit excessive iodide-induced strong mitochondrial superoxide production; however, diethyldithiocarbamic acid (DETC, 2 mM) can further increase excessive iodide-induced mitochondrial superoxide production. By using transmission electron microscopy (TEM), we noted accumulated myelinoid bodies with lipid droplets and numerous apoptotic nuclear bodies at 24 h in FRTL cells. In addition, we demonstrat...Continue Reading

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Citations

Mar 26, 2014·Thyroid : Official Journal of the American Thyroid Association·Panayota Kolypetri, George Carayanniotis
Nov 10, 2014·European Journal of Nutrition·Malgorzata Karbownik-LewinskaAndrzej Lewinski
Jul 28, 2016·Journal of Environmental Radioactivity·F SudbrockK Schomäcker
Jan 31, 2017·Oxidative Medicine and Cellular Longevity·Tingting WangXiaomei Yao
Oct 31, 2020·Journal of the Endocrine Society·Tomomi KurashigeYuji Nagayama

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