Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells

Journal of Pharmacopuncture
Eun-A ChoiHwa-Seung Yoo

Abstract

Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs). IS4 concentration inhibited the production of interleukin-1beta (IL-1 β), interleukin-6 (IL-6), and granulocyte -macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50㎍/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF- α) stimulated by LPS. IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.

References

Jan 15, 1998·Journal of the National Cancer Institute·Z YaoJ Konishi
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·D K Nordstrom, C N Alpers
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Seiko IshidaIra Herskowitz
Mar 17, 2007·The Journal of Nutritional Biochemistry·Nanmei LiuMaria C Linder
Jun 11, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Brian G BlairStephen B Howell
Aug 26, 2009·The Journal of Experimental Medicine·Akihiro KimuraTadamitsu Kishimoto
May 11, 2010·Future Medicinal Chemistry·Arnab Gupta, Svetlana Lutsenko

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
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SPSS

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